Temperature Dependence of Testes Intersticium Cell Membrane Permeability for Cryoprotectant Molecules
Keywords:permeability coefficients, activation energy, testis intersticium cells, volumetry, cryoprotectants
Osmotic behavior of the rat testis intersticium cells in cryoprotectants solutions â€“ substances belonging to alcohols (ethylene glycol (EG), glycerol and 1,2-butane diol (1,2-BD)), oxides (dimethyl sulfoxide (Me2SO)) and amides (dimethyl formamide (DMFA)) was studied. Permeability coefficients of testis cells for these cryoprotectants were determined at 35, 20 and 5Â°C by volumetric analysis and the Kedem-Kachalsky modified physico-mathematical model. The calculated values of the activation energies for EG, glycerol, 1,2-BD, Me2SO and DMFA molecules are 53.28, 46.92, 36.33, 58.89, 62.99 kJ/mol, respectively. Testis cell membranes were established to be equally well permeable for EG, glycerol, 1,2-BD and Me2SO, and their permeability for DMFA at 35Â°C was comparable with that for water molecules.
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Copyright (c) 2020 N. A. Chernobai, A. V. Pakhomov, I. F. Kovalenko, G. A. Bozhok, S. Ye. Kovalenko, L. F. Rozanov
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