Osmotic Properties of Erythrocytes Frozen in Media Containing Non-Penetrating and Penetrating Cryoprotectants

Authors

  • V. V. Ramazanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. A. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocytes, combined cryopreservatives, osmotic hemolysis

Abstract

Osmotic hemolysis of erythrocytes frozen in combined crypreservatives containing polymer non-penetrating (dextran, PEG) and penetrating (DMSO, glucose) cryoprotectants was studied. Erythrocytes frozen with the polymers within the range of NaCl concentrations 0.45–0.9% are characterized by lower osmotic resistance than intact cells and by higher osmotic resistance within the range of NaCl concentrations 0.09–0.4%. The higher the polymer concentration in the freezing medium is, the higher resistance is. Combination of non-penetrating and penetrating cryoprotectants in cryopreservatives maintains normal osmotic resistance of frozen erythrocytes. The results obtained allow presuming that enhancement in osmotic resistance of erythrocytes frozen within the range of NaCl concentrations 0.09–0.4% is associated with a growth in polymer concentration gradient on cell membranes in the process of cooling. Combination a penetrating cryoprotectant with a polymer in freezing media promotes a positive correction of cryoprotective efficiency of the latter owing to weakening osmotic stress during freezing, which is caused by concentration of the non-penetrating polymer cryoprotectant.

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Published

2010-04-19

How to Cite

Ramazanov, V. V., & Bondarenko, V. A. (2010). Osmotic Properties of Erythrocytes Frozen in Media Containing Non-Penetrating and Penetrating Cryoprotectants. Problems of Cryobiology and Cryomedicine, 20(1), 47–58. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/216

Issue

Vol. 20 No. 1 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. V. Ramazanov, V. A. Bondarenko

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