Diffusion of Poly-Atom Alcohols and Their Methoxyderivatives via Rat and Rabbit Erythrocyte Membranes

Authors

  • G. V. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Koshchiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryoprotectants, methoxyderivatives of alcohols, membrane permeability, protein channels, animal erythrocytes

Abstract

There were determined the coefficients for membrane permeability of rat and rabbit native erythrocytes for glycerol, ethylene glycol molecules, their methoxyderivatives at 25 and 37°C, as well as erythrocytes, incubated with mercury sulfhydryl reagent (pCMBS): the band 3 protein blocker at 25°C. The activation energy of the process of cryoprotectant molecule transfer through erythrocyte membranes at 25 and 37°C and after incubation with pCMBS was calculated. The rate of substance diffusion into rat erythrocytes was shown to be higher, than in rabbit ones. As an example the coefficient of EG permeability into rat and rabbit erythrocytes at 37°C is equal to 13.95x10-6 and 5.21x10-6 m/sec, correspondingly. The rate of transmembrane diffusion of EG and glycerol methoxyderivatives is 50–80 times higher, compared to EG and glycerol. There was proposed and discussed in details the transport mechanism of studied substances through rat and rabbit erythrocyte membranes.

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Published

2009-12-23

How to Cite

Kovalenko, G. V., Kovalenko, I. F., Linnik, T. P., & Koshchiy, S. V. (2009). Diffusion of Poly-Atom Alcohols and Their Methoxyderivatives via Rat and Rabbit Erythrocyte Membranes. Problems of Cryobiology and Cryomedicine, 19(4), 413–420. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/230

Issue

Vol. 19 No. 4 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 G. V. Kovalenko, I. F. Kovalenko, T. P. Linnik, S. V. Koshchiy

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