Temperature Effect on Erythrocyte Membrane Permeability for Cryoprotectants with Different Hydrophobicities
Keywords:erythrocytes, cryoprotectants, permeability, activation energy
There were measured the permeability coefficients for native and pCMBS-treated human erythrocytes for 1,2-propane diol (1,2-PD) and dimethyl sulfoxide (DMSO) within 3â€“37Â°C temperature range and the values of activation energy for these cryoprotectantsâ€™ penetration within certain temperature ranges were determined. Erythrocyte cooling below physiological temperatures was shown to result in thermotropic changes of membrane state. The most pronounced changes in activation energy were observed within 27â€“30 and 12Â°C range. Sharp decrease in activation energy of penetration of 1,2-PD hydrophilic cryoprotectant within 30â€“37Â°C range disappears after erythrocyte treatment with pCMBS, testifying to a significant contribution of 1,2-PD protein hydrophilic channels into a transmembrane flow. The presence of kinks in Arrhenius plots and an increase in activation energy of penetration of both hydrophilic 1,2-PD and hydrophobic DMSO within temperature range below 12Â°C is not eliminated via erythrocyte treatment with sulphhydryl reagent. Thus, these effects can not be explained by closing the protein pathways of permeability for studied substances and they are
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Copyright (c) 2020 E. V. Davydova, O. I. Gordienko
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