Platelet Cryopreservation. 1. Development of Cryopreservative Solutions, Based on Cryoprotectant Combination and Study of Their Efficiency

Authors

  • O. A. Bogdanchikova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

platelets, cryopreservation, combined cryoprotective solutions, aggregation, hypotonic shock reaction, platelet clot retraction

Abstract

Cryoprotective efficiency of cryopreservative solutions, based on combining different cryoprotectants during platelet freezing, was studied in the research. There were established the cryoprotectant combinations such as: 10% dimethyl acetamide (DMAC)/1,2-propane diol (1,2-PD), DMAC/glycerol and DMAC/oxyethylated glycerol (n = 5), significantly increasing the integrity of cryopreserved platelets, compared to the results of freezing under protection of monocryoprotective media, containing one of the mentioned above substances, including 10% dimethyl sulfoxide.

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Published

2009-11-30

How to Cite

Bogdanchikova, O. A., & Kompaniets, A. M. (2009). Platelet Cryopreservation. 1. Development of Cryopreservative Solutions, Based on Cryoprotectant Combination and Study of Their Efficiency. Problems of Cryobiology and Cryomedicine, 19(3), 324–337. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/256

Issue

Vol. 19 No. 3 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 O. A. Bogdanchikova, A. M. Kompaniets

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