Peculiarities of Cryopreservation Effect on Functional Potential of Fetal Liver Hemopoietic Stem Cells of Various Gestation Terms

Authors

  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. G. Dubrava Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. Ye. Yampolskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. A. Sirous Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fetal liver cells, cryopreservation, hemopoietic precursors

Abstract

The capability of cryopreservation to modify structural and functional status of bioobject, including fetal liver cells (FLCs), may be applied for cell and tissue therapy optimisation. The efficiency of FLCs application is determined by the presence in them of a wide spectrum of cell populations, including hemopoietic stem cells. There has been established a change in phenotypic and functional characteristics of FLCs with gestation term prolongation, manifesting in a decrease in their colony-forming potential, CD34+ number and a simultaneous increase in Mac-1+ and LFA-1+ cells. Cryopreservation was shown as capable for a selective enrichment of 18 gestation day’s FLCs by hemopoietic precursors with a high proliferative potential. This fact may be determining one in their therapeutic effect manifestation.

Downloads

Published

2009-07-06

How to Cite

Goltsev, A. N., Dubrava, T. G., Ostankova, L. V., Grischenko, V. I., Yampolskaya, E. Y., Ostankov, M. V., Bondarovich, N. A., & Sirous, M. A. (2009). Peculiarities of Cryopreservation Effect on Functional Potential of Fetal Liver Hemopoietic Stem Cells of Various Gestation Terms. Problems of Cryobiology and Cryomedicine, 19(2), 186–199. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/273

Issue

Vol. 19 No. 2 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. N. Goltsev, T. G. Dubrava, L. V. Ostankova, V. I. Grischenko, E. Ye. Yampolskaya, M. V. Ostankov, N. A. Bondarovich, M. A. Sirous

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).