Protective Effect of Methylcellulose during Cryopreservation of Inoculated Cells in Serum-Free Medium
Keywords:
inoculated cells, cryopreservation, preservation medium, methylcelluloseAbstract
There was experimentally shown a possibility to replace blood serum for methylcellulose (MC) in the media for cryopreservation of inoculated cells. Combination of 0.1% MC with 5% dimethyl sufoxide (DMSO) in the preservation media provided the highest indices of post-thaw cell survival. Freeze-thawing of cells in the medium contained MC, but without DMSO resulted in a significant reduction of survived cells. The cells, which were pre-cultured in the medium with 0.5% fetal serum (FS) and 0.1% MC, showed higher survival post freeze-thawing in serum-free medium comparing to the cells, which we pre-cultured in the medium with 10% FS. Probl Cryobiol Cryomed 2013; 23(2):116–123.
References
Vysekantsev I.P., Martsenyuk V.F., Anan'ina A.E. et al. Cryopreservation of industrial and collection strains of microorganisms. In: Actual Problems of Cryobiology and Cryomedicine / Ed. by A.N. Goltsev Kharkov, 2012: 401–440.
Golubev D.B., Solomin A.A., Medvedeva M.N. Guidelines for application of cell cultures in virology Moscow: Meditsina, 1976.
Koschiy S.V., Vysekantsev I.P., Petrenko T.F. Proliferation and cryopreservation of SPEV cells, cultured in the medium with low content of serum. Biofizika Zhyvoy Kletki 2008; 9: 70–71.
Cryopreservation of cell suspensions. Ed. by A.A. Tsutsaeva Kiev: Naukova Dumka, 1983 240 p.
Culture of animal cells. Methods. Ed. by R. Freshney. Moscow: Mir, 1989.
Laboratory investigation methods in clinic: Manual. Ed. by V.V. Menshikov. Moscow: Meditsina, 1987.
Prisedsky Yu.G. Statistical processing of results of biological experiments: Manual. Donetsk, 1999.
Ukraine Patent N58224 IPC C12 N5/2. Method of adaptation of adhesive cells to growth in serum-free media. S.V. Koschiy, A.M. Goltsev, I.P. Vysekantsev; Filed 13.08.2010; Publ. 11.03.2011. Bull. N7.
Medzon E.L., Merchant D.J. Interaction of the LM cell surface with methylcellulose and vaccinia virus. Mode of action and implications for large scale vaccine production. In Vitro 1971; 7 (1): 46–58.
Merchant D.J., Hellman K.B., Schneider H. et al. Protection of animal cells with methylcellulose. Bact. Proc 1962: 141.
Merten O.W., Petres S. and Couve E. A simple serum-free freezing medium for serum-free cultured cells. Biologicals 1995; 23: 185–189.
Nair L.S, Laurencin C.T. Polymers as biomaterials for tissue engineering and controlled drug delivery. Adv Biochem Eng Biotechnol 2006; 102: 47–90.
Onho T., Kurita K., Abe S. et al. A simple freezing medium for serum-free cultured cells. Cytotechnology 1988; 1: 257–260.
Sannino A., Demitri C., Madaghiele M. Biodegradable cellulose-based hydrogels: design and applications. Materials 2009; 2: 353–373.
Tate M.C., Shear D.A., Hoffmann S.W. et al. Biocompatibility of methylcellulose-based constructs designed for intracerebral gelation following experimental traumatic brain injury. Biomaterials 2001; 22 (10): 1113–1123.
Thirumala S., Gimble J.M., Devireddy R.V. Evaluation of methylcellulose and dimethyl sulfoxide as the cryoprotectants in a serum-free freezing media for cryopreservation of adipose–derived adult stem cells. Stem Cells Dev 2010; 19 (4): 513–522.
Yoshida T., Takeuchi M. Primary culture and cryopreservation of mouse astrocytes under serum-free conditions. Cytotechnology 1991; 5 (2): 99–106.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Svetlana V. Koschiy, Anatoliy N. Goltsev, Igor P. Vysekantsev, Valentina F. Martsenyuk, Tatyana M. Gurina, Larisa V. Sokol
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
