Permeability of SPEV Cell Membranes for Water and DMSO Molecules

Authors

  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Koschiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. V. Timofeyeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Sakun Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. Ye. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

permeability coefficients, SPEV cells, volumetry

Abstract

Using the volumetry method there were defined the permeability coefficients of SPEV cell membranes for water and DMSO. It has been established that with increasing the volumes of cell dimensions their membrane permeability for DMSO molecules reduces. Numeric modeling of osmotic behavior of SPEV cells during freezing in DMSO presence has shown that during cooling with 1°C/min the dehydration of SPEV cells occurs within the temperature range of –7.5...–1°C and the rise of cooling rate up to 5°C/min and higher may be the cause of intracellular crystallization.

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Published

2009-05-25

How to Cite

Kovalenko, I. F., Koschiy, S. V., Timofeyeva, E. V., Sakun, A. V., Kovalenko, S. Y., Vysekantsev, I. P., & Rozanov, L. F. (2009). Permeability of SPEV Cell Membranes for Water and DMSO Molecules. Problems of Cryobiology and Cryomedicine, 19(1), 25–31. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/283

Issue

Vol. 19 No. 1 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 I. F. Kovalenko, S. V. Koschiy, E. V. Timofeyeva, A. V. Sakun, S. Ye. Kovalenko, I. P. Vysekantsev, L. F. Rozanov

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