Study of Antigen Expression by Human Neurocells of Different Gestation Term during Culturing In Vitro

Authors

  • M. I. Lisyanyy Institute of Neurosurgery named after Acad. A.P. Romodanov of Academy of Medical Sciences of Ukraine, Kiev
  • L. D. Lyubich Institute of Neurosurgery named after Acad. A.P. Romodanov of Academy of Medical Sciences of Ukraine, Kiev
  • V. M. Semenova Institute of Neurosurgery named after Acad. A.P. Romodanov of Academy of Medical Sciences of Ukraine, Kiev
  • L. P. Stayno Institute of Neurosurgery named after Acad. A.P. Romodanov of Academy of Medical Sciences of Ukraine, Kiev
  • M. S. Vysotskiy Institute of Neurosurgery named after Acad. A.P. Romodanov of Academy of Medical Sciences of Ukraine, Kiev
  • A. I. Potapova Institute of Neurosurgery named after Acad. A.P. Romodanov of Academy of Medical Sciences of Ukraine, Kiev

Keywords:

neurocells, culturing, antigens

Abstract

The HLA expression by human neurocells (NC) of different gestation term and differentiation on the protein and mRNA levels using methods of immunophenotyping and RT-PCR has been studied. Under culturing human NC of 5–6 week’ gestation term in DMEM + retinol acetate the amount and percentage of HLA I and II expressing cells on the protein and mRNA levels were decreased whereas the presence in the culturing medium of the growth factors contributed to preservation (FGF) or increasing (EGF) of positive expressing cells amount. Under culturing in DMEM the HLA I and II expressing Bulbus olfactorius cells number decreased from day 10th to 20th.

Downloads

Published

2008-12-22

How to Cite

Lisyanyy, M. I., Lyubich, L. D., Semenova, V. M., Stayno, L. P., Vysotskiy, M. S., & Potapova, A. I. (2008). Study of Antigen Expression by Human Neurocells of Different Gestation Term during Culturing In Vitro. Problems of Cryobiology and Cryomedicine, 18(4), 486–489. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/358

Issue

Vol. 18 No. 4 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 M. I. Lisyanyy, L. D. Lyubich, V. M. Semenova, L. P. Stayno, M. S. Vysotskiy, A. I. Potapova

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).