Cord Blood Preservation Method with No Washing-Out: Estimation of Populations of Nucleated Cells
Keywords:
nucleated cells, cryopreservation, PEO-1500, populations, cord bloodAbstract
There were elaborated new effective methods for isolation and cryopreservation of cord blood nucleated cells, which allow to preserve quite whole quantitative and qualitative contents of nucleated cells (including hemopoietic cells) after isolation and demonstrate quite a high recovery after freeze-thawing. The analysis of CD45+-cell population content testified that CD34+-cells as well as lymphocytes and monocytes manifested a high level of recovery and viability after freezing.
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Copyright (c) 2020 L. A. Babiychuk, P. M. Zubov, V. V. Ryazantsev, O. L. Zubova
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