Effect of Hypothermic Storage of Isolated Neural Cells of Newborn Rats in Media of Different Composition on Its Behavior in Culture
Keywords:
hypothermic storage, neural cells, newborn rats, culturing, multicellular aggregatesAbstract
The behavior of isolated neural cells of newborn rats in culture after hypothermic storage either in sucrose-based solution or saline medium supplemented with 10% serum or serum-free was studied. Hypothermic storage in both saline medium DMEM/F12 and sucrose-based solution was shown to be effective in preserving the populations of differentiated and stem/progenitor cells for 2 days. It was demonstrated that one day of hypothermic storage in medium DMEM/F12 either supplemented with serum or serum-free, as well as in serum-free sucrose-based solution did not effect the structural-functional state of differentiated and stem/progenitor neural cells of newborn rats.
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Copyright (c) 2020 M. V. Shevchenko, A. N. Sukach
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