Effect of Hypothermic Storage of Isolated Neural Cells of Newborn Rats in Media of Different Composition on Its Behavior in Culture

Authors

  • M. V. Shevchenko G.S. Skovoroda Kharkov National Pedagogical University, Kharkov
  • A. N. Sukach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

hypothermic storage, neural cells, newborn rats, culturing, multicellular aggregates

Abstract

The behavior of isolated neural cells of newborn rats in culture after hypothermic storage either in sucrose-based solution or saline medium supplemented with 10% serum or serum-free was studied. Hypothermic storage in both saline medium DMEM/F12 and sucrose-based solution was shown to be effective in preserving the populations of differentiated and stem/progenitor cells for 2 days. It was demonstrated that one day of hypothermic storage in medium DMEM/F12 either supplemented with serum or serum-free, as well as in serum-free sucrose-based solution did not effect the structural-functional state of differentiated and stem/progenitor neural cells of newborn rats.

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Published

2012-12-20

How to Cite

Shevchenko, M. V., & Sukach, A. N. (2012). Effect of Hypothermic Storage of Isolated Neural Cells of Newborn Rats in Media of Different Composition on Its Behavior in Culture. Problems of Cryobiology and Cryomedicine, 22(3), 423–432. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/47

Issue

Section

Theoretical and Experimental Cryobiology