Effect of Cooling Rate under Various Temperature Intervals on Survival of Steroidogenic Potential of Suspension of Interstitial Cells of Adult Rat’s Testes

Authors

  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

testosterone, Leydig cells, cooling rate, 3β-hydroxysteroid dehydrogenase, overcooling

Abstract

The effect of cryopreservation conditions (cryoprotectant concentration, cell incubation with cryoprotectants prior to freezing, cooling of samples with various temperature intervals) on survival of steroidogenic cells in the testscular interstitium cell suspension (ICS) has been investigated. This resulted in designing of cryopreservation protocol for ICS, which provided the highest survival and functional activity of steroidogenic cells if compared with the protocols already existing for testicular biological material.

Downloads

Published

2007-12-15

How to Cite

Gurina, T. M., Pakhomov, A. V., & Bozhok, G. A. (2007). Effect of Cooling Rate under Various Temperature Intervals on Survival of Steroidogenic Potential of Suspension of Interstitial Cells of Adult Rat’s Testes. Problems of Cryobiology and Cryomedicine, 17(4), 394–402. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/486

Issue

Vol. 17 No. 4 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. M. Gurina, A. V. Pakhomov, G. A. Bozhok

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).