Ultrastructure of Nucleated Cells of Cord Blood and Blood of Adult Donors at Different Stages of Cryopreservation. Report 1. The Changes on the Stage of Cells Isolation
Keywords:nucleated cells, adult donor blood, cord blood, ultrastructure
Ultrastructure characteristics were studied in the nucleated cells from adult donor blood and cord blood isolated by different methods. Electron microscopical investigation revealed quite good preservation of characteristic ultrastructure in nucleated cells of adult donor blood and cord blood isolated by two-step centrifugation and sedimentation in Poliglyukin. Presence of large amount of ribosomes and polysomes in cytoplasm testifies to a high activity of synthesizing and reparative function of the cells. Isolation of nucleated cells from adult donor blood and cord blood using Ficoll leads to destructions of membrane structures and organelles, which impedes the restoration of normal metabolic activity by intracellular reparation.
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Copyright (c) 2020 L. A. Babiychuk, R. K. Migunova, O. F. Nevzorova, V. P. Nevzorov
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