PEO-1500 Inhibits Human Erythrocyte Ca2+-ATPase

Authors

  • N. G. Zemlyanskikh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Nikolchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocytes, Ca2 -ATPase, cryopreservation, cryoprotectant, polyethylene oxide

Abstract

Human erythrocyte Ca2+-ATPase provides the maintenance and regulation of intercellular calcium level. Change in its functional activity under cryopreservation plays an important role in cell survival and structural integrity preservation. PEO-1500 exocellular cryoprotectant inhibits erythrocyte Ca2+-ATPase activity at incubation stage but the following freeze-thawing cycle (–196...37°C) in its presence does not affect the settled regimen of this enzyme functioning. The inhibition caused by PEO-1500 effect has a reversible character and the erythrocyte Ca2+-ATPase activity is recovered after its dilution in cell suspension that is essential for cryopreserved cell normal functioning in blood bed after transfusion. The inhibition of Ca2+-ATPase activity is apparently associated to physical and chemical membrane modification under this compound effect.

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Published

2007-06-15

How to Cite

Zemlyanskikh, N. G., Nikolchenko, A. Y., & Babijchuk, L. A. (2007). PEO-1500 Inhibits Human Erythrocyte Ca2+-ATPase. Problems of Cryobiology and Cryomedicine, 17(2), 115–125. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/511

Issue

Vol. 17 No. 2 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. G. Zemlyanskikh, A. Yu. Nikolchenko, L. A. Babijchuk

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