PEO-1500 Inhibits Human Erythrocyte Ca2+-ATPase
Keywords:erythrocytes, Ca2 -ATPase, cryopreservation, cryoprotectant, polyethylene oxide
Human erythrocyte Ca2+-ATPase provides the maintenance and regulation of intercellular calcium level. Change in its functional activity under cryopreservation plays an important role in cell survival and structural integrity preservation. PEO-1500 exocellular cryoprotectant inhibits erythrocyte Ca2+-ATPase activity at incubation stage but the following freeze-thawing cycle (â€“196...37Â°C) in its presence does not affect the settled regimen of this enzyme functioning. The inhibition caused by PEO-1500 effect has a reversible character and the erythrocyte Ca2+-ATPase activity is recovered after its dilution in cell suspension that is essential for cryopreserved cell normal functioning in blood bed after transfusion. The inhibition of Ca2+-ATPase activity is apparently associated to physical and chemical membrane modification under this compound effect.
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Copyright (c) 2020 N. G. Zemlyanskikh, A. Yu. Nikolchenko, L. A. Babijchuk
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