Cryoresistance of Organotypic Cultures Derived from Adrenal Tissues of Rats of Various Ages

Authors

  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. Kh. Rumiekh V.N. Karazin Kharkov National University, Kharkov

Keywords:

cryopreservation, adrenal organotypic cultures, hormone secretion, viability, animal’s age

Abstract

The paper covers the studies in cryosensitivity of organotypic cultures derived from the adrenal tissues of white rats of various ages (1, 3, 6, 12 and 24 months ) based on the analysis of viable cells in culture prior to and after cryopreservation in 5% dimethyl sulfoxide presence. It has been established that secreting ability of culture cells depends on an age of the animal that the tissues were derived from. The cultures of tissues of aged animals (12-24 months) have lower activity and higher sensitivity to low temperature effect.

Downloads

Published

2007-06-15

How to Cite

Bondarenko, T. P., & Rumiekh, Y. K. (2007). Cryoresistance of Organotypic Cultures Derived from Adrenal Tissues of Rats of Various Ages. Problems of Cryobiology and Cryomedicine, 17(1), 44–49. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/530

Issue

Vol. 17 No. 1 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. P. Bondarenko, Yu. Kh. Rumiekh

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).