Pretreatment with Ozone Elevates the Resistance of Saccharomyces cerevisiae Yeast Membranes during Freeze-Thawing
Keywords:Saccharomyces cerevesiae, freeze-thawing, ozone, cross adaptation, oxidative stress, adaptive response
Ð¢he effect of ozone on injury of Saccharomyces cerevisiae yeast membranes following freezing (down to â€“196Â°C) and warming (up to 30Â°C) was investigated using fluorescent staining and flow cytometry. It has been shown that freeze-thawing of S. cerevisiae cells in physiological solution resulted in the injury of membranes in 65.6% of total cells population. Treatment of the cells with ozone in a dose of 29â€“240 Ñ€mol Ðž3/106 cells prior to freezing allowed to reduce significantly the number of damaged cells: (16.1 Â± 0.9)% of total population. The observed effect was explained by cross-adaptation caused by ozone introduction: the adaptive response to slight oxidative stress initiated the genetically determined mechanisms of responces to other stresses, including the one caused by freeze-thawing.
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