Pretreatment with Ozone Elevates the Resistance of Saccharomyces cerevisiae Yeast Membranes during Freeze-Thawing

Authors

  • Vasiliy D. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Irina P. Goryachaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Irina A. Buryak Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Igor P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo24.01.038

Keywords:

Saccharomyces cerevesiae, freeze-thawing, ozone, cross adaptation, oxidative stress, adaptive response

Abstract

Тhe effect of ozone on injury of Saccharomyces cerevisiae yeast membranes following freezing (down to –196°C) and warming (up to 30°C) was investigated using fluorescent staining and flow cytometry. It has been shown that freeze-thawing of S. cerevisiae cells in physiological solution resulted in the injury of membranes in 65.6% of total cells population. Treatment of the cells with ozone in a dose of 29–240 рmol О3/106 cells prior to freezing allowed to reduce significantly the number of damaged cells: (16.1 ± 0.9)% of total population. The observed effect was explained by cross-adaptation caused by ozone introduction: the adaptive response to slight oxidative stress initiated the genetically determined mechanisms of responces to other stresses, including the one caused by freeze-thawing.


Probl Cryobiol Cryomed 2014; 24(1):38–46.

Author Biographies

Vasiliy D. Zinchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Irina P. Goryachaya, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryobiophysics

Irina A. Buryak, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long Temperature Preservation of Biological Objects at Low Temperatures and Microbiology

Igor P. Vysekantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Long Temperature Preservation of Biological Objects at Low Temperatures and Microbiology

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Published

2014-03-25

How to Cite

Zinchenko, V. D., Goryachaya, I. P., Buryak, I. A., & Vysekantsev, I. P. (2014). Pretreatment with Ozone Elevates the Resistance of Saccharomyces cerevisiae Yeast Membranes during Freeze-Thawing. Problems of Cryobiology and Cryomedicine, 24(1), 38–46. https://doi.org/10.15407/cryo24.01.038

Issue

Section

Theoretical and Experimental Cryobiology