Role of Overcooling in Preserving Clonogenic Properties of Fibroblast-Like Progenitor Cells of Human Fetal Liver After Cryopreservation
Keywords:cryopreservation, fibroblast-like progenitor cells, human fetal liver
Effect of different freezing conditions on human fetal liver (HFL) cell viability and colony-forming activity of HFL fibroblast-like progenitor cells (FPCs) was investigated. This progenitor type was established to be a high sensitive to a damaging effect of overcooling. Overcooling reduction at a slow three-step freezing enables a successful cryopreservation of FPCs in primary suspension of HFL cells with preservation of their clonogenic properties.
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Copyright (c) 2020 N. G. Skorobogatova, V. P. Grischuk, A. Yu. Petrenko
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