Role of Overcooling in Preserving Clonogenic Properties of Fibroblast-Like Progenitor Cells of Human Fetal Liver After Cryopreservation

Authors

  • N. G. Skorobogatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. P. Grischuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, fibroblast-like progenitor cells, human fetal liver

Abstract

Effect of different freezing conditions on human fetal liver (HFL) cell viability and colony-forming activity of HFL fibroblast-like progenitor cells (FPCs) was investigated. This progenitor type was established to be a high sensitive to a damaging effect of overcooling. Overcooling reduction at a slow three-step freezing enables a successful cryopreservation of FPCs in primary suspension of HFL cells with preservation of their clonogenic properties.

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Published

2006-03-31

How to Cite

Skorobogatova, N. G., Grischuk, V. P., & Petrenko, A. Y. (2006). Role of Overcooling in Preserving Clonogenic Properties of Fibroblast-Like Progenitor Cells of Human Fetal Liver After Cryopreservation. Problems of Cryobiology and Cryomedicine, 16(1), 24–31. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/613

Issue

Vol. 16 No. 1 (2006): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. G. Skorobogatova, V. P. Grischuk, A. Yu. Petrenko

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