Influence of Duration of a Moderate Low Temperature Storage on RNA of Cattle Diarrhea Virus Strains

Authors

  • M. Yu. Stegniy National University of Pharmacy, Kharkov
  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • B. T. Stegniy Institute of Experimental and Clinical Veterinary of the Ukrainian Academy of Agrarian Sciences, Kharkov

Keywords:

diarrhea virus, moderate low temperatures, polymerase chain reaction

Abstract

There was studied the effect of storage duration under conditions of moderate low temperature (from –18 down to –30°C) on the possibility to change nucleotide sequence of their genome. Analysis of obtained results testify to the fact that within the most conservative site (15’-untranslated region) of cattle diarrhea virus genome the nucleotide sequences are not impaired both in case of virus storage at temperatures from –18 down to –30°C for 13 months and under longer storage term (for 12 years). In reaction with outer primers there was impossible to reveal the products of polymerase chain reaction and when using inner primers with the length of 195 pairs of nucleotides they were obtained, that confirmed the RNA degradation during storage of moderate low temperatures.

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Published

2006-03-31

How to Cite

Stegniy, M. Y., Goltsev, A. N., & Stegniy, B. T. (2006). Influence of Duration of a Moderate Low Temperature Storage on RNA of Cattle Diarrhea Virus Strains. Problems of Cryobiology and Cryomedicine, 16(1), 76–81. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/619

Issue

Vol. 16 No. 1 (2006): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 M. Yu. Stegniy, A. N. Goltsev, B. T. Stegniy

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