Succinate-Dehydrogenase and Glucose-6-Phosphate Dehydrogenase Activities of Human Sperm at Low Temperature Preservation Stages

Authors

  • V. L. Rodionova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. V. Nikitchenko V.N. Karazin Kharkov National University, Kharkov
  • N. N. Tchoob Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Cherepanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

human sperm, cryopreservation, glucose-6-phosphatedehydrogenase, succinate dehydrogenase

Abstract

We have carried out the research in glucose-6-phosphatedehydrogenase (G6PDG) and succinate dehydrogenase (SDG) activities in human sperm following the incubation with cryoprotective medium (glycerol-lactose-yolk) and after hypothermic storage and freezing down to liquid nitrogen temperature. At the low temperature steps preservation the activities were characterized with slight differences at selected cooling regimens. Statistically significant variation in SDG and G6PDG activities was demonstrated during samples’ storage at –12°C both with and without cryoprotective medium.

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Published

2006-06-15

How to Cite

Rodionova, V. L., Nikitchenko, Y. V., Tchoob, N. N., & Cherepanov, V. V. (2006). Succinate-Dehydrogenase and Glucose-6-Phosphate Dehydrogenase Activities of Human Sperm at Low Temperature Preservation Stages. Problems of Cryobiology and Cryomedicine, 15(2), 207–211. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/634

Issue

Vol. 15 No. 2 (2005): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 V. L. Rodionova, Yu. V. Nikitchenko, N. N. Tchoob, V. V. Cherepanov

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