Study of Effect of Some Permeable Cryoprotectants on Microsomes Using Chemiluminescence Method

Authors

  • E. V. Onischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

liver microsomes, cryoprotectants, lipid peroxidation, free-radical processes, chemiluminescence

Abstract

The effect of freezing and low molecular cryoprotectants on the intensity of peroxidative processes in rat’s liver microsomes have been studied using chemiluminescence (CL) method. As cryoprotectants (CP) we used solutions of glycerol (Gl), 1,2-propane diol (1,2-PD) and DMSO, prepared on Tris-buffer within the concentration range from 5 to 40% (w/w). The values of spontaneous chemiluminescence (SCL), amplitude, H2O2 light sum and Fe2+-induced CL were noted to augment when the cryoprotectant content in microsome suspension was higher than 30% (w/w).

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Published

2005-03-22

How to Cite

Onischenko, E. V., & Zinchenko, A. V. (2005). Study of Effect of Some Permeable Cryoprotectants on Microsomes Using Chemiluminescence Method. Problems of Cryobiology and Cryomedicine, 15(1), 50–55. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/645

Issue

Vol. 15 No. 1 (2005): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 E. V. Onischenko, A. V. Zinchenko

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