Gander Sperm Cryopreservation in Plastic Straws

Authors

  • S. V. Bychko Institute for Poultry Research of the Ukrainian Academy of Agrarian Sciences, Borki, Kharkov Region
  • O. B. Artemenko Institute for Poultry Research of the Ukrainian Academy of Agrarian Sciences, Borki, Kharkov Region
  • O. V. Tereshchenko Institute for Poultry Research of the Ukrainian Academy of Agrarian Sciences, Borki, Kharkov Region
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

gander sperm, cooling regimen, plastic straws, spermatozoa integrity

Abstract

The authors determined the major zones of spermatozoa injuries during cryopreservation and the optimum rates for gander semen cooling at 0 to -196°C in plastic straws. At the initial stage of freezing down to –10°C the semen cooling was shown to be reasonable with the rate of 5°C/min, 50°C/min at further cooling –10 to –90°C, and –90 to –196°C with the cooling rate of 15°C/min. Morphological integrity of gander sex cells when freezing according to the method proposed was found to be 10-15% higher comparing to the standard one elaborated at the Institute for Poultry Research.

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Published

2004-12-15

How to Cite

Bychko, S. V., Artemenko, O. B., Tereshchenko, O. V., & Linnik, T. P. (2004). Gander Sperm Cryopreservation in Plastic Straws. Problems of Cryobiology and Cryomedicine, (4), 61–66. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/662

Issue

No. 4 (2004): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 S. V. Bychko, O. B. Artemenko, O. V. Tereshchenko, T. P. Linnik

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