Cell Sensitivity of Recultured Cellular SPEV Line and Candida albicans Fungi to Extra- and Intracellular Crystallisation Processes

Authors

  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. F. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Koschij Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

crystal formation, freezing, yeast-like fungi, cryoprotectants, cooling rate, cryomicroscopy

Abstract

The freeze-thawing processes of SPEV cell suspensions and C. albicans fungi were investigated with a polarizing-interference microscopy. The SPEV cells were found out to be highly sensitive to an intracellular crystal formation, which depended in a considerable extent on the level of cell suspension supercooling prior to crystallisation beginning in an extracellular medium. C.albicans cells are more resistant to initiation of an intracellular crystal formation, that provides them a higher cryoresistance.

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Published

2004-09-15

How to Cite

Rozanov, L. F., Vysekantsev, I. P., Petrenko, T. F., Kovalenko, I. F., & Koschij, S. V. (2004). Cell Sensitivity of Recultured Cellular SPEV Line and Candida albicans Fungi to Extra- and Intracellular Crystallisation Processes. Problems of Cryobiology and Cryomedicine, (3), 18–25. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/670

Issue

No. 3 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 L. F. Rozanov, I. P. Vysekantsev, T. F. Petrenko, I. F. Kovalenko, S. V. Koschij

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