Cooling, Cryopreservation and Gene Expression in Mammalian Cells

Authors

  • B. Fuller Royal Free & University College Medical School, London
  • C. Green Northwick Park Institute for Medical Research, London
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy

Keywords:

low temperatures, hypothermia, cryopreservation, gene products, stress response

Abstract

Altered gene expression in response to cooling has been identified in many prokaryotic organisms, plants and lower invertebrates such as insects, but the effects in mammalian cells are less clearly understood. This review was undertaken to identify responses of mammalian cells to cold temperatures, such as might be encountered in therapeutic procedures where body temperatures are lowered, in preservation of cells and organs, and in cryopreservation. In general, cold elicits a range of stress responses through identified signaling pathways, which may determine the survival or otherwise of the cells. Under conditions of mild hypothermia, there is evidence for responses which reflect an ordered acclimation to the new environment, whilst deep cooling invokes a more general stress response. The concepts reported here form part of a presentation to the Society for Low Temperature Biology (2002, London) Meeting Chromosomes, Genes and Cryobiology

Downloads

Published

2004-09-15

How to Cite

Fuller, B., Green, C., & Grischenko, V. I. (2004). Cooling, Cryopreservation and Gene Expression in Mammalian Cells. Problems of Cryobiology and Cryomedicine, (3), 58–71. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/676

Issue

No. 3 (2004): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 B. Fuller, C. Green, V. I. Grischenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).