Effect of Low Temperature Storage on Steroid Hormones Content Stability in Human Oocyte Follicular Fluid
Keywords:follicular fluid of human oocyte, estradiol, progesterone, testosterone, low temperature storage
There was studied the estradiol (E), progesterone (P) and testosterone (T) content in follicular fluid (FF) of human oocyte preovulatory follicles, native and low-temperature-stored one (-196Â°C). We have compared the effect of two freezing regimens (one-step immersion into liquid nitrogen and a two-step one with the preliminary exposure in nitrogen vapors) on the stability of concentration maintenance of these hormones in FF for 1 and 6 months. Stability of estradiol, progesterone and testosterone was shown to be kept within the whole observation period and to be independent upon the FL freezing regimen selected.
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Copyright (c) 2020 E. A. Yakovleva, A. G. Gerodes
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