Effect of Cryopreservation Using Slow Freezing or Vitrification on Viability and Metabolic Activity of Mesenchymal Stromal Cells Encapsulated Within Alginate Spheres with Diameter of 1 mm and More
DOI:
https://doi.org/10.15407/cryo24.03.222Keywords:
mesenchymal stromal cells, alginate microspheres, vitrification, multicomponent cryoprotective solution, stepwise exposureAbstract
The aim of the study was to evaluate the effect of exposure duration of alginate encapsulated mesenchymal stromal cells (MSCs) in multicomponent cryoprotective solution DEPS-1 (10% dimethyl sulfoxide, 20% ethylene glycol, 1,2-propane diol and
0.5 M sucrose) on survival and metabolic activity following cryopreservation using vitrification. The spheres of 1mm diameter obtained by dropwise addition of sodium alginate into calcium chloride were used in the experiments. Alginate spheres derived by this method contained more cells as compared to the spheres of (0.5 ± 0.2) mm derived by pulverizing the alginate into calcium chloride. It was shown that using of multicomponent cryoprotective solution DEPS-1 allowed achieving successful cryopreservation of MSCs encapsulated in alginate spheres of different sizes. It was found that saturation with cryoprotective solution of MSCs encapsulated in spheres with 1.2 mm size needed a longer exposure with CPA if compared with spheres of 0.6 mm size.
Probl Cryobiol Cryomed 2014; 24(3):222-230.
References
Katsen-Globa A., Meiser I., Petrenko Y.A. et al. Towards ready-to-use 3-D scaffolds for regenerative medicine: adhesion-based cryopreservation of human mesenchymal stem cells attached and spread within alginate-gelatin cryogel scaffolds. J Mater Sci Mater Med. 2014; 25(3): 857–871. CrossRef PubMed
Murua A., Portero A., Orive G. et al. Cell microencapsulation technology: towards clinical application. J Control Rel 2008; 132(4): 76–83. CrossRef PubMed
Pittenger M.F., Mackay A.M., Beck S.C. et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284(5411): 143–147. CrossRef PubMed
Petrenko A.Y., Petrenko Y.A., Mazur S.P. et. al. Isolation and multilinear differentiation of stromal cells from fetal and adult tissue. Transplantology 2007; 9(1): 218–220.
Pravdyuk A.I., Petrenko Y.A., Volkova N.A., Petrenko A.Y. Properties of human mesenchymal stromal cells in alginate microcapsules. Biotekhnologiya 2010; 3(2): 62–70.
Pravdyuk A.I., Petrenko Y.A., Fuller B.J., Petrenko A.Y. Cryopreservation of alginate encapsulated mesenchymal stromal cells. Cryobiology 2013; 66(3): 215–222. CrossRef PubMed
Zaikov V.S., Petrenko Y.A., Trufanova N.A. et. al. Cryopreservation of human mesenchymal stromal cells in suspension and encapsulated in alginate microbeads by vitrification in multicomponent solutions. Probl Cryobiol Cryomed 2012; 22(4): 14–20.
Zimmermann H., Shirley S.G., Zimmerman U. Alginate-based encapsulation of cells: past, present and future. Cur Diabetes Rep 2007; 7(4): 314–320. CrossRef
Wagh V., Meganathan K., Jagtap S. et al. Effects of cryopreservation on the transcriptome of human embryonic stem cells after thawing and culturing. Stem Cell Rev 2011; 7: 506–517. CrossRef PubMed
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Vedeney S. Zaikov, Yuriy A. Petrenko, Natalia A. Trufanova, Aleksey I. Pravdyuk, Natalia A. Volkova, Svetlana P. Mazur, Aleksandr Y. Petrenko
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
