Liver Structure in Rats with Experimental Hepatic Failure Following Implantation of Macroporous Carrier Seeded with Cryopreserved Fetal Liver Cells
DOI:
https://doi.org/10.15407/cryo24.04.292Keywords:
liver, alginate-gelatin matrices, cryopreserved fetal liver cells, hepatic failure model, implantationAbstract
The study demonstrates the state of bioconstructs based on cryopreserved fetal liver cells (FLC), seeded into macro-porous alginate-gelatin matrices (AGM), as well as the effect of the bioconstructs implantation on the liver structure in hepatic failure rat models. As carriers for rat FLC the wide-porous cryogel AGM were applied. The additional alginate capsule formed around the AGM prevented the colonization of matrices by the recipient cells in vivo and did not affect the viability and expansion of FLC in vitro. The structure of bioconstructs was maintained during at least 28 days post implantation. The experimental liver damage in rats was modeled by the injection of the hepatocyte proliferation inhibitor 2-acetylaminofluorene together with the partial hepatectomy, that led to pronounced changes in liver structure (disorderin of trabecular structure of the parenchyma and ductular reaction within the portal and periportal areas). The implantation of FLC-seeded AGM additionally covered by the alginate shell into greater omentum of rats with hepatic failure led to organotypic recovery of pathologically altered liver structure. It was shown that the alginate capsule around the AGM provided isolation for seeded cells, and FLC within the wide-porous AGM had a therapeutic effect when implanted in rats with liver failure.
Probl Cryobiol Cryomed 2014; 24(4):292-301.
References
Dolle L., Best J., Mei J. et al. The quest for liver progenitor cells: A practical point of view. J Hepatol 2010; 52(1): 117–129. CrossRef PubMed
Fuller B.J., Petrenko A.Yu., Rodriguez J.V. et al. Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification. CryoLetters 2013; 34(4): 432–452.
Glicklis R., Shapiro L., Agbaria R. et al. Hepatocyte behavior within three-dimensional porous alginate scaffolds. Biotechnology and Bioengineering 2000; 67(3): 344–353. CrossRef
Levit R.D., Landazuri N., Phelps E.A. et al. Cellular encapsulation enhances cardiac repair. J American Heart Association 2013; 2(5): 1–11. CrossRef PubMed
Petrenko A.Yu., Sukach A.N. Isolation of intact mitochondria and hepatocytes using vibration. Analytical Biochem 1991; 194(2): 326–332. CrossRef
Petrenko Yu.A., Ivanov R.V., Lozinsky V.I, Petrenko A.Yu. Comparison of the methods for seeding human bone marrow mesenchymal stem cells to macroporous alginate cryogel carriers. Cell Technologies in Biology and Medicine 2010; (4): 225–228.
Petrenko Yu.A., Ivanov R.V., Petrenko A.Yu., Lozinsky V.I. Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells. J Mater Sci: Mater Med 2011; 22(6): 1529–1540. CrossRef PubMed
Petrenko Y.A., Jones D.R.E., Petrenko A.Y. Cryopreservation of human fetal liver hematopoietic stem / progenitor cells using sucrose as an additive to the cryoprotective medium. Cryobiology 2008; 57(3): 195–200. CrossRef PubMed
Ping Zhou, Lessa N., Estrada D.C. et al. Decellularized liver matrix as a carrier for transplantation of human fetal and primary hepatocytes in mice. Liver Transpl 2011; 17(4): 418–427. CrossRef PubMed
Skorobogatova N.G., Novikov A.N., Fuller B.J., Petrenko A.Yu. Importance of a three-stage cooling regime and induced ice nucleation during cryopreservation on colony-forming potential and differentiation in mesenchymal stem/progenitor cells from human fetal liver. CryoLetters 2010; 31(5): 371–379.
Soltys K.A., Soto-Gutierrez A., Nagaya M. et al. Barriers to the successful treatment of liver disease by hepatocyte transplantation. J Hepatol 2010; 53(4): 769–774. CrossRef PubMed
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Dmitriy V. Gritsay, Alexander S. Lebedinskiy, Olga V. Ochenashko, Yuriy A. Petrenko, Viktoriya V. Volina, Natalya. A. Volkova, Alexander Yu. Petrenko
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
