Foxp3 Gene Expression Value in Regulatory T Cells in Pathogenesis of Graft-Versus-Host Disease Induced with Cryopreserved Allogenic Material
Keywords:graft-versus-host disease, regulatory T cells, Foxp3 gene, cryopreserved bone marrow
Content of regulatory T cells (Treg) and their Foxp3 gene expression level in the recipients with graft-versus-host disease (GVHD) induced by introduction of fresh aspirated or cryopreserved allogenic bone marrow combined with lymph node cells were under study in this research. In GVHD experimental model the deficiency in Tregwas shown to play an important role in maintaining autoimmune process. In the animals with GVHD induced with fresh aspirated allogenic bone marrow (BM) almost twofold decrease in both Tregcontent and the expression level of Foxp3 gene were established as compared to the syngeneic BM recipients. An increased level of Foxp3 gene expression under the maximum reduction of their content in the animals with GVHD, induced with cryopreserved allogenic BM introduction, was noted. Cryopreservation of allogenic BM, used for GVHD induction, reduced its immune reactivity and, as a result, clinical evidence of pathology. Our findings enable to broaden the notion about the mechanisms of immune conflicts development in GVHD both at cellular and molecular levels.
Probl Cryobiol Cryomed 2014; 24(4):322-331.
Chen X., Vodanovic-Jankovic S., Johnson B. et al. Absence of T-cell control of Th1 and Th17 cells is responsible for the autoimmune-mediated pathology in chronic graft-versus-host disease. Blood 2007; 110(10): 3804â€“3813. CrossRef PubMed
Goltsev A.N., Lutsenko E.D., Ostankova L.V. et al. Application of cryobiology in solving immune-conflict problems when grafting a histoincompatible bone marrow. Problems of Cryobiology 1996; (2): 3â€“10.
Goltsev A.N., Matsevitaya I.Yu., Lutsenko E.D. et al. On the modification of immunoreactivity of myelotransplant after cryopreservation. Problems of Cryobiology 2010; 20(2): 145â€“152.
Herrington C., McGee J., editors. Molecular clinical diagnostics. Methods. Moscow: Mir; 1999.
Korsunsky I.A., Rumyantsev A.G., Bykovskaya S.N. Role of regulatory T-cells of CD4+CD25+ and mesenchymal stem cells of bone marrow in suppressing graft-versus-host response. Onkogematologiya 2008; (3): 45â€“51.
Kozlov V.A., Yarilin A.A., Ametov A.S. et al. Natural regulator T-cells and bound with them cytokines under chronic autoimmune thyroiditis. Immunologiya 2008; (6): 357â€“361.
Manko V.M., Petrov R.V. Transplantology and tissue engineering of hematopoietic stem cells. Factors controlling their functions. Allergologiya i Immunologia 2008; 9(4): 413â€“427.
McGeachy M.J., Stephens L.A., Anderton S.M. Natural recovery and protection from autoimmune encephalomyelitis: Contribution of CD4+CD25+ regulatory cells within the central nervous system. J Immunol 2005; 175(5): 3025â€“3032. CrossRef PubMed
Sakaguchi S., Sakaguchi N., Asano M. et al. Immunologic cell-tolerance maintained by activated T cells expressing IL-2 receptor a-chains (CD25). J Immunol 1995; 155(3): 1151â€“1164. PubMed
Shevelev A.S. Graft-versus-host response and transplantation disease. Moscow: Meditsina; 1976.
Yarilin A.A., Donetskova A.D. Natural regulatory T cells and FOXP3 factor. Immunologiya 2006; 27(3): 176â€“188.
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Copyright (c) 2020 Anatoliy N. Goltsev, Tatuyana G. Dubrava, Yuliya A. Gayevskaya, Elena D. Lutsenko, Maxim V. Ostankov, Irina Yu. Matsevitaya
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