Cryopreserved Cord Blood Preparations in Combined Surgical Treatment of Purulent Complications of Type II Diabetes mellitus


  • Yaroslav O. Popovych Ivano-Frankivsk City Clinical Hospital Nr. 1, Ivano-Frankivsk



pyoinflammatory complications of diabetes, metabolic changes, endotoxicosis, preparations of cryopreserved cord blood, correction of homeostasis


Diabetic foot syndrome (DFS) is a serious late complication of Diabetes mellitus (DM) and the unsolved problem of endocrine surgery. This pathology occurs in 80% of patients with diabetes and half of them leads to an extremity amputation. Combined drug and surgical treatment of patients with type II diabetes complications with pyonecrotic DFS stages and sacrococcygeal and perineum phlegmons was analysed. There were used the medical immune biological preparations (MIBP) based on cryopreserved placental blood which contribute to the normalization of immunological reactions, proliferation processes, stimulate a reparation, possess antioxidant, adaptogenic, anti-hypoxic, anabolic and anti-inflammatory effects. The patients were administered with "Cryocell-Cryocord", i.e. placental blood serum (108 patients), and in severe endointoxication they were additionally treated within "Cryocell-Hemocord", placental blood leukoconcentrate (37 patients). When applying MIBP the insulin dosage is reduced by 25-30%. A significant decrease in leukocyte index, the level of an average molecular weight peptides is observed. Cytoarchitecture of erythrocytes significantly improved. Potentiation of the preparations on the activity of metalloenzymes, hepatocyte cytolysis enzymes was found. The number of proteases inhibitors decreases with a parallel increase in the content of albumin, total protein amount, normalization of organ specific enzymes.The increase in total content of immunoglobulins, a sharp decline in the level of circulating immune complexes (antigen-antibody autoantigens) with a parallel increase in the antibodies of natural protection were revealed. In addition, under the influence of cord blood MIBP the wound cleansing and its healing starts faster, pyo-inflammatory process is restricted at the foot level, lower leg edema disappears. The data obtained can be interpreted as recovery of reparative processes in the wound due to effective correction of metabolic-homeostatic changes in the patient suffering from diabetes and its complications.

Probl Cryobiol Cryomed 2014; 24(4):332-345.


Babaei-Jadidi R., Karachalias N., Ahmed. N. et al. Prevention of incipient diabetic nephropathy by high-dose thiamine and benfotiamine. Diabetes 2003; 52(8): 2110–2120. CrossRef PubMed

Babijchuk L.O., Grischenko V.I., Gurina T.M. et al., inventors. Cryopreservation way for cord blood nucleated cells, including hematopoietic stem cells. Patent of Ukraine N92227, IPC A01N1/02.

Berchun V.V., Bobyriova L.E., Bobyriov V.M., Berchun N.V., inventors. Way for a combined therapy of diabetic foot syndrome. Patent of Ukriane N45675A, IPC A61K 35/50, A61B 17/00, A61P 3/10, A61P 17/02.

Boulton A.J.M. The diabetic foot: from art to science. The 18th Camillo Golgi lecture. Diabetologia 2004; 47(8): 1343–1353. CrossRef PubMed

Davis W.A., Norman P.E., Bruce D.G., Davis T.M.E. Prеdictors, consequences and costs of diabetes-related lower extremity amputation complicating type 2 diabetes: The Fremantle Diabetes Study. Diabetologia 2006; 49: 2634–2641. CrossRef PubMed

Fedorenko V.P., Pavlovskiy M.P. Efficiency of Berlithion and Vazoprostan application in a combined surgical treatment of necrotic-inflammatory foot lesions in patients with diabetes mellitus. Surgery of Ukraine 2005; 13(1): 58–61.

Goltsev A.N., Kalinichenko T.A. Human umbilical cord blood as a source of hemopoietic cells for clinical application. Part I. Nature of hemopoietic potential. Problems of Cryobiology 1998; (1): 3–21.

Goltsev K.A., Ovsyannikov S.Ye., Kozhina O.Yu., Ostankov M.V., Goltsev A.N. Correction of metabolic impairments with 'Cryocell-Hemocord' cord blood preparation during acute purulent peritonitis. Problems of Cryobiology 2011; 21(1): 96–103.

Grischenko V.I. Achievements and prospects of cryobiology development in Ukraine. Problems of Cryobiology 2005; 15(3): 231–244.

Gryschenko V.I., Lipina O.V., Prokopyuk O.S., Moshko Yu.O., inventors. Biostimulating preparation 'Cryocord-C' and the way for treating salpingoophoritis with its applicaiton. Patent of Ukraine N42454, IPC A61K 35/16, A61P 31/00.

Jeffcoe W.J., Von Houtum W.H. Amputation as a marker of the quality of foot care in diabetes. Diabetologia 2004; 47(12): 2051–2058. CrossRef PubMed

Kaliman V.P. The role of glycosylated hemoglobin and albumin in diagnosis of hyperglycemic states. Experimental and Clinical Medicine 2008; (1): 53–55.

Komarov F.I., Korovkin B.F., Menshikov V.V. Biochemical studies in clinic. Elista: APP Dzhangar; 2001.

Meschishin I.F., Pishak V.P., Grigorieva N.P. Basics of metabolism and energy. Chernivtsi: Medical University; 2005.

Ovchinnikova O.V., Bondarenko I.A., Lazurenko V.V. et al. Application of cryopreserved cord blood in treatment of hypoferric anemia in pregnants. Problems of Cryobiology 2008; 18(3): 361–363.

Pavlenko I.A., Chepkiy L.P., Ivanyushko O.V., Ilchishin R.O. Endogenic intoxication and its correction under craniocerebral injure. Ukrainian Neurosurgical Journal 2008; (8): 68–71.

Popovych Ya.O. Combined surgical treatment of purulent-necrotic lesions in diabetes mellitus with the use of 'Cryocord C'. Klinichna Khirurgiya 2006; (11–12): 87.

Rai N.K., Ansari M., Shukla V.K., Thipathi K. Effect of glycaemic control on apoptosis in diabetic wounds. J Wound Care 2005; 14 (6): 277–281. CrossRef PubMed

Shidlovsky V.O., Deikalo I.M., Chepil I.V. Immune-correcting therapy in combined treatment of patients with severe purulent pathologies. Problems of Cryobiology 2005; 15(1): 79–84.

Smykodub O.I., Novytska A.V. Therapy of patients with type 2 diabetes mellitus in the disease onset by embryonic stem cells. Transplantologiia 2007; 9(1): 278–282.

Tronko N.D. Analysis, priorities, ways of implementation of the State Target Program 'Diabetes Mellitus' during 2009–2013. Proceedings of the Ist Congress of Association of Endocrinologists in Ukraine 'New Technologies in Diabetology'; Zdorov'ia Ukrainy 2010; 247(18): 42–43.

Turchyn I.S., Larin O.S., Drozdovych I.I. et al. The use of combined organ cultures of endocrine glands for the treatment of endocrinopathy: achievements and prospects. Transplantologiya 2007; 9(1): 293–295.

Vasilyuk S.M. Diabetic foot syndrome. Ivano-Frankivsk: DKD; 2005. PubMed




How to Cite

Popovych, Y. O. (2014). Cryopreserved Cord Blood Preparations in Combined Surgical Treatment of Purulent Complications of Type II Diabetes mellitus. Problems of Cryobiology and Cryomedicine, 24(4), 332–345.



Cryomedicine, Clinical and Experimental Transplantology