Effect of brain and liver extracts on outcome of hypothermic storage of newborn rat derived neural cells

Authors

  • Mariya V. Shevchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Aleksandr N. Sukach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

DOI:

https://doi.org/10.15407/cryo25.01.013

Keywords:

newborn rats, neural cells, brain extract, liver extract, hypothermic storage, culturing

Abstract

The behavior of isolated neural cells of newborn rats in culture after hypothermic storage in sucrose-based solution and saline medium DMEM/F-12 in the presence of serum or brain and liver extracts was studied. Hypothermic storage in saline medium DMEM/F-12 in the presence of serum or liver and brain extracts and sucrose-based solution without serum or in the presence of brain extract allows to preserve the differentiated and stem/progenitor cells populations for 2 days. The hypothermic storage of cells for more than this period results in their death. During the hypothermic storage in DMEM/F-12 the brain/liver extracts together with the serum augment the survival rate for newborn rats neural cells, and under hypothermic storage in sucrose-based solution the brain and liver extracts cause positive and negative effects, respectively, on neural cell survival. It was found that the brain and liver extracts allow to effectively substitute the serum during the hypothermic storage of neural cells in DMEM/F-12 medium.


Probl Cryobiol Cryomed 2015; 25(1): 13-23.

Author Biographies

Mariya V. Shevchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

Aleksandr N. Sukach, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

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Published

2015-03-20

How to Cite

Shevchenko, M. V., & Sukach, A. N. (2015). Effect of brain and liver extracts on outcome of hypothermic storage of newborn rat derived neural cells. Problems of Cryobiology and Cryomedicine, 25(1), 13–23. https://doi.org/10.15407/cryo25.01.013

Issue

Section

Theoretical and Experimental Cryobiology