Efficiency of the Sucrose-Based Solution and UW Solution for Hypothermic Storage of Human Mesenchymal Stromal Cells in Suspension or Within Alginate Microspheres

Authors

  • Dmitriy N. Tarusin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Yuriy A. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Olga A. Semenchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Vitaliy V. Mutsenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Vedeney S. Zaikov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Aleksandr Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

DOI:

https://doi.org/10.15407/cryo25.04.329

Keywords:

mesenchymal stromal cells, alginate microspheres, hypothermic storage, preservation solution, culture, adhesion, metabolic activity, induced differentiation

Abstract

The effect of hypothermic storage (HS) on viability and functional activity of human mesenchymal stromal cells (MSCs) in suspension and within alginate microspheres (AMSs) was studied. Cells were stored in sealed cryovials at 4°C in culture medium (CM), sucrose-based solution (SBS) and that of the University of Wisconsin (UW). After 3 and 7 days of storage the viability, metabolic activity and morphology of MSCs were assessed by MTT-assay, Alamar Blue test, adhesive activity, morphology and ability of multilineage differentiation at monolayer culture conditions. Hypothermic storage of MSCs suspension in CM for 3 days was established to result in a 3–4-fold decrease in their viability and adhesive ability. The encapsulation into AMSs partially prevented cell death. Hypothermic storage for 7 days caused death of almost all MSCs both in suspension and AMSs. The substitution of CM for UW and SBS preservation solutions maintained the MSCs viability and metabolic activity following HS within 3 days both in suspension and within AMSs. After 7 days of HS in UW and SBS solutions the MSCs in suspensions and within AMSs preserved their viability on the level of 60–80%, adhesive properties, metabolic activity, as well as the ability for induced adipogenic and osteogenic differentiation. The use of UW and SBS preservation solutions enabled to prolong the hypothermic storage terms for MSCs.


Probl Cryobiol Cryomed 2015; 25(4):329-339.

Author Biographies

Dmitriy N. Tarusin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

Yuriy A. Petrenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

Olga A. Semenchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

Vitaliy V. Mutsenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

Vedeney S. Zaikov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

Aleksandr Yu. Petrenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiochemistry

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Published

2015-12-21

How to Cite

Tarusin, D. N., Petrenko, Y. A., Semenchenko, O. A., Mutsenko, V. V., Zaikov, V. S., & Petrenko, A. Y. (2015). Efficiency of the Sucrose-Based Solution and UW Solution for Hypothermic Storage of Human Mesenchymal Stromal Cells in Suspension or Within Alginate Microspheres. Problems of Cryobiology and Cryomedicine, 25(4), 329–339. https://doi.org/10.15407/cryo25.04.329

Issue

Section

Theoretical and Experimental Cryobiology