Effect of Cryopreservation Regimens on Behavior of SPEV Cells in Culture
Keywords:SPEV cells, adhesion, cryopreservation
This work demonstrates the ability of using the substrate-adhered SPEV cells as the object for studying the kinetics of osmotic reactions when exposing them to cryoprotective media. Permeability coefficients of SPEV cell membranes for the molecules of water and dimethyl sulfoxide (DMSO) have been determined. Using the physical-mathematical model for cell suspension freezing and calculated permeability coefficients the osmotic SPEV cell behavior during cryopreservation with different cooling rates has been simulated. The experiments on freezing the SPEV cell suspension showed that the cooling rate of 1.5 deg/min could ensure the optimal protection of the cells against damage in 1M DMSO presence. It has been demonstrated that the cooling rate above and below the optimum may cause a cell death as the result of intracellular crystallization or dehydration.
Probl Cryobiol Cryomed 2016; 26(2):116-123.
Curtis A.S.G., Forrester J.V., Mcinnes C., Lawrie F. Adhesion of cells to polystyrene surfaces. J. Cell Biol 1983; 97: 1500â€“1506. CrossRef
Farrant J. Mechanism of cell damage during freezing and thawing and its prevention. Nature 1965; 205(4978): 1284â€“1289. CrossRef
Gordienko E.A., Pushkar N.S. Physical grounds of low-temperature preservation for cell suspensions. Kiev: Naukova dumka; 1994.
Kovalenko I.F., Koschiy S.V., Timofeeva E.V. et al. Permeability of SPEV cell membranes for water and DMSO molecules. Problems of Cryobiology 2009; 19(1): 25â€“31.
Orlov A.I. Applied Statistics. Moscow: Ekzamen; 2006.
Schipitsyn M.A., Dmitrieva E.A., Voropaeva O.V. Investigation of influence of incubation medium composition and substrate on adhesion of erythroblastic islands. Modern High Technologies 2006; 3: 44.
Sergeev V.A., Sobko Y.A. Cell culture in veterinary and biotechnology. Kiev: Urozhay; 1990.
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Copyright (c) 2020 Igor F. Kovalenko, Svetlana Ye. Kovalenko, Igor P. Vysekantsev, Tatyana F. Petrenko, Leonid F. Rozanov, Nadezhda A. Chernobay
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