Growth Factors BDNF And TGF1β in Tissue of Cryopreserved Human Placental Amniotic Membrane after Antiglaucomatous Surgery

Authors

  • Irina L. Kazmiruk Kharkov Medical Academy of Post-Diploma Education, Kharkov
  • Yuriy A. Demin Kharkov Medical Academy of Post-Diploma Education, Kharkov
  • Vladimir V. Ryazantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo26.02.145

Keywords:

human placental amniotic membrane, cryopreservation, permeability, cell growth factors, antiglaucomatous surgery

Abstract

The effect of cryopreserved human placental amniotic membrane (HPAM) on the release of cell growth factors BDNF and TGF1β into both an environment and eye within the observation period of up to 21 days has been studied. By enzyme immunoassay there was investigated the content of cell growth factors in ocular tissues after use as a coating of the frozen according two-step regimen HPAM in antiglaucomatous surgery. The efficiency of using the frozen-thawed HPAM in respect of releasing the cell growth factors after its application into the post-surgery defect zone has been demonstrated. There was found the dependence of accumulation of growth factors TGF1β and BDNF on time of cryopreserved HPAM application, eye tissue type and tropism of growth factors.

 

Probl Cryobiol Cryomed 2016; 26(2):145-154.

Author Biography

Vladimir V. Ryazantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryocytology

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Published

2016-06-23

How to Cite

Kazmiruk, I. L., Demin, Y. A., & Ryazantsev, V. V. (2016). Growth Factors BDNF And TGF1β in Tissue of Cryopreserved Human Placental Amniotic Membrane after Antiglaucomatous Surgery. Problems of Cryobiology and Cryomedicine, 26(2), 145–154. https://doi.org/10.15407/cryo26.02.145

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology