Effect of DMSO and Its Decay Products on Spermatozoa and Survival of Loach Misgurnus fossilis L. Embryos

Authors

  • Yu. E. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • E. F. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • O. V. Bibenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • A. A. Zinchenko National Centre of Medicinal Means of the Ministry of Health Care of the Ukraine, Kharkiv

Abstract

To examine the effect of dimethylsulfoxide (DMSO) of different purity on the genome of reproductive cells, spermatozoa from loach (Misgurnus fossilis L.) were equilibrated in 3 media containing 10 % DMSO of different purity at 4°C for 40-50 minutes. Survival of embryos derived from sperm subjected to DMSO exposure was not affected by the purity of DMSO fractions before 20‑th stage of embryo development. It was equally decreased for all three studied fractions of DMSO and it was 1/3 times lower than that in the control. It was explained by presence of cytotoxic and selective effects of DMS0 on sperm population in the ejaculate. The estimated decrease of embryo survival after 20-th stage for the embryos derived from sperm equilibrated with fraction I and II correlated with the data obtained after spectrophotometrical analysis of admixtures in DMSO solution.  The decrease of embryo survival after 20-th stage was interpreted as indirect evidence of genetic disturbance caused by equilibration of sperm with fraction I and II. The equilibration of sperm with the purest (III) fraction of DMSO caused decrease of survival of embryos as well but not for all females. It was explained by less significant effect of the purest fraction of DMSO on the spermatozoa, and by variety in repair capability and genetic potential between different female individuals. The survival of embryos was dependent on the stage of development. Thus, DMSO has a cytotoxic and selective effect as well as causes some disturbance in genetic material of sperm that consequently brings to the decease of embryo survival. The estimated effect was dependent on the purity of DMSO, individual female, egg quality and stage of embryo development.

Downloads

Published

2002-12-25

How to Cite

Kopeika, Y. E., Grischenko, V. I., Kopeika, E. F., Linnik, T. P., Bibenko, O. V., & Zinchenko, A. A. (2002). Effect of DMSO and Its Decay Products on Spermatozoa and Survival of Loach Misgurnus fossilis L. Embryos. Problems of Cryobiology and Cryomedicine, (4), 45–56. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/988

Issue

No. 4 (2002): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 Yu. E. Kopeika, V. I. Grischenko, E. F. Kopeika, T. P. Linnik, O. V. Bibenko, A. A. Zinchenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).