Assessment of Phagocytic Activity of Leukoconcentrate Neutrophils Cryopreserved with Dimethyl Acetamide after Post-Thaw Rehabilitation in Medium Containing Cord Blood Low-Molecular Fraction (Below 5 kDa)

Authors

  • A. K. Gulevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. S. Akhatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. N. Moiseeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. L. Gorina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Chizhevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Stepanyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cattle cord blood low-molecular fraction, cryopreservation, neutrophils, rehabilitating medium

Abstract

The influence of the cattle cord blood low-molecular fraction (below 5 kDa) on the functional activity of leukocytes after cryopreservation was studied. The stimulating effects of the fraction (0.15 mg/ml) on digesting and engulfing activities of frozenthawed neutrophils were observed. It was established that the usage of dimethyl acetamide (5%) as a cryoprotectant allowed preserving up to 80% of viable leukocytes.

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Published

2024-05-21

How to Cite

Gulevsky, A. K., Akhatova, Y. S., Moiseeva, N. N., Gorina, O. L., Chizhevsky, V. V., & Stepanyuk, L. V. (2024). Assessment of Phagocytic Activity of Leukoconcentrate Neutrophils Cryopreserved with Dimethyl Acetamide after Post-Thaw Rehabilitation in Medium Containing Cord Blood Low-Molecular Fraction (Below 5 kDa). Problems of Cryobiology and Cryomedicine, 22(1), 71–78. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/99

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. K. Gulevsky, Yu. S. Akhatova, N. N. Moiseeva, O. L. Gorina, V. V. Chizhevsky, L. V. Stepanyuk

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