Assessment of Phagocytic Activity of Leukoconcentrate Neutrophils Cryopreserved with Dimethyl Acetamide after Post-Thaw Rehabilitation in Medium Containing Cord Blood Low-Molecular Fraction (Below 5 kDa)
Keywords:cattle cord blood low-molecular fraction, cryopreservation, neutrophils, rehabilitating medium
The influence of the cattle cord blood low-molecular fraction (below 5 kDa) on the functional activity of leukocytes after cryopreservation was studied. The stimulating effects of the fraction (0.15 mg/ml) on digesting and engulfing activities of frozenthawed neutrophils were observed. It was established that the usage of dimethyl acetamide (5%) as a cryoprotectant allowed preserving up to 80% of viable leukocytes.
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Copyright (c) 2020 A. K. Gulevsky, Yu. S. Akhatova, N. N. Moiseeva, O. L. Gorina, V. V. Chizhevsky, L. V. Stepanyuk
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