@article{Plaksina_Sidorenko_Bozhok_2017, title={Cryopreservation of Multicellular Spheroids Derived From Newborn Piglet Adrenal Glands}, volume={27}, url={https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1353}, DOI={10.15407/cryo27.04.322}, abstractNote={<p>Application of special in vitro culture techniques for the cells, derived from different animal and human organs, makes<br />possible the obtaining of multicellular spheroids (MSs), being the natural 3-D environment for cells unlike the standard culture with the<br />cells in monolayer. Previously we have shown that MSs formed in the newborn piglet adrenal cell culture are capable to produce the<br />neuroblast-like cells, expressing the neuronal marker β-III-tubulin. In the present work we have assessed the regimens for MSs<br />cryopreservation using 5, 7 and 10% dimethyl sulfoxide (DMSO) and 25% fetal bovine serum (FBS). Analysis of some characteristics<br />of cryopreserved MSs (surface adhesion, capability to produce the neuroblast-like cells and monolayer formation by fibroblast-like<br />cells) allowed to choose the cryopreservation regimen with 1°C/min cooling rate in the presence of 10% DMSO as the most optimal<br />one. The FBS supplement to the medium did not significantly affect the cryopreservation outcome, although there was found a<br />tendency to increase the capability of cryopreserved MSs to produce the neuroblast- and fibroblast-like cells.</p><p><em>Probl Cryobiol Cryomed 2017; 27(4): </em><em>322-333</em></p>}, number={4}, journal={Problems of Cryobiology and Cryomedicine}, author={Plaksina, Ekaterina M. and Sidorenko, Olga C. and Bozhok, Galina A.}, year={2017}, month={Dec.}, pages={322–333} }