@article{Goltsev_Volina_Sokol_Ostankov_Goltsev_Chernousova_Kozhina_2010, title={Effect of Hypothermic Storage and Cryopreservation on Human Cord Blood Leucoconcentrate Cells}, volume={20}, url={https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/190}, abstractNote={<p>Pre-cryopreservation hypothermic storage and cryopreservation process itself effects on integrity, viability and membrane properties of hemopoietic stem cells with high proliferative activity and self-support potentials as well as of non-hemopoietic human cord blood leucoconcentrate (HCBL) cells were investigated, as well as correlation between phenotypic marker expression and functional condition of these cells was assessed. The data obtained allowed experimental substantiating the maximally acceptable term of hypothermic storage (24 hrs) of cord blood prior to production of HCBL. A rise in plasmatic membrane permeability of stem hemopoietic cells with a high proliferative activity potential after their cryopreservation was shown. The permeability also increased, as the term of preliminary hypothermic storage was prolonged.</p>}, number={3}, journal={Problems of Cryobiology and Cryomedicine}, author={Goltsev, A. N. and Volina, V. V. and Sokol, L. V. and Ostankov, M. V. and Goltsev, K. A. and Chernousova, S. S. and Kozhina, O. Yu.}, year={2010}, month={Sep.}, pages={303–308} }