@article{Voyda_Biryukova_Vysekantsev_2012, title={Effect of Cryopreservation and Storage under Mineral Oil on Pheno- and Genotype Features of Escherichia coli Clinical Strains Isolated from Different Biotopes}, volume={22}, url={https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/50}, abstractNote={<p>The differences between the spectra of sensitivity to antibiotics of <em>Escherichia coli </em>strains isolated from different biotopes were found. It was found that storage at –196°С during 5 years (observation term) of bacteria frozen under protection of 10% dimethyl sulfoxide solution did not change the spectrum of antibiotic resistance. After storage under the layer of mineral oil at the temperature of 6...8 °С and subculturing some strains lost their resistance to beta-lactams, that was probably due to the loss of specific plasmids. </p>}, number={4}, journal={Problems of Cryobiology and Cryomedicine}, author={Voyda, Yu. V. and Biryukova, S. V. and Vysekantsev, I. P.}, year={2012}, month={Dec.}, pages={462–467} }