TY - JOUR AU - Tamarina, I. V. AU - Bozhok, G. A. AU - Gurina, T. M. AU - Gubina, N. F. AU - Bondarenko, T. P. PY - 2012/03/14 Y2 - 2024/03/29 TI - Cryopreservation of Newborn Mice Adrenal Cell Suspension: 1. Effect of DMSO Concentration as a Component of Cryoprotective Medium JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 22 IS - 1 SE - Theoretical and Experimental Cryobiology DO - UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/100 SP - 79-87 AB - <p>Influence of DMSO concentrations as a component of cryopreservative medium on viability, survival and functional activity of the newborn mice adrenal cells after freeze-thawing cycle has been established in this study. Maximal values of viability (63.7; 66.7%) and survival (78.3; 95.7%) are observed after freezing of newborn mice adrenal cell suspension (ACS) in the medium with 15 and 20% DMSO. Regardless the high values of ACS viability and survival after freeze-thawing cycle, none of the DMSO concentrations being used was not capable to preserve proper post-thaw cell proliferation and hormone production.</p> ER -