TY - JOUR AU - Gulevsky, Aleksandr K. AU - Tretiak, Diana V. PY - 2016/12/23 Y2 - 2024/03/29 TI - Features of Protein-Peptide and Carbohydrate Composition of Supernatants From Tenebrio Molitor Larvae After Cold Acclimation JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 26 IS - 4 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo26.04.322 UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1241 SP - 322-330 AB - <p>The paper describes the chromatographic studies on the molecular-mass distribution of proteins and peptides of supernatants, derived from <em>Tenebrio molitor </em>larvae during cold acclimation. Supernatants of non-acclimated larvae of <em>T. molitor </em>had the highest amount of peptide fractions. It has been shown that cold-acclimated <em>T. molitor </em>larvae comprised the low-molecular peptide fractions with MW of (540 ± 20) – (2.255 ± 85) Da, and high-molecular peptides with MW of (4.675 ± 225) – (6.595 ± 550) Da were characteristic for non-acclimated larvae. The content of sugars and polyols in supernatants of cold-acclimated and non-acclimated <em>T. molitor </em>larvae was determined. It has been found that glucose concentration in non-acclimated <em>T. molitor </em>larvae was in 1.34 times higher than in cold-acclimated ones. Аcclimated <em>T. molitor </em>larvae had more hydrophilic and less hydrophobic proteins as compared to non-acclimated ones. As well it has been established that non-acclimated <em>T. molitor </em>larvae had low concentrations of polyols (sorbitol and mannite), which were absent in acclimated insects.</p><p><em>Probl Cryobiol Cryomed 2016; 26(4): 322–330</em><em></em></p> ER -