TY - JOUR AU - Ivchenko, Tetyana V. AU - Vitsenya, Tatyana I. AU - Shevchenko, Nadiya A. AU - Bashtan, Natalia O. AU - Kornienko, Sergiy I. PY - 2017/06/25 Y2 - 2024/03/28 TI - Hypothermic and Low-Temperature Storage of Garlic (Allium sativum L.) for in Vitro Collections JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 27 IS - 2 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo27.02.110 UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1291 SP - 110-120 AB - <p><span id="tinymce" class="mceContentBody " dir="ltr">New methods to establish various types of plant genetic collection are developed in Ukraine in order to preserve<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> the existing species and varieties. This research deals with the effects of short-term hypothermic (4°C) and long-term low<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> temperature (–196°C) storage on viability of the collection samples of garlic (<span style="font-family: Arial,Italic; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;">Allium sativum L.<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;">). In the first case the culture of garlic<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> sprout of Duchess variety was stored <span style="font-family: Arial,Italic; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;">in vitro <span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;">at the temperature of 4°C in the Murashige-Skoog (MS) medium with different mineral<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> composition (1/2 MS and MS) in the dark for 3, 6, 9 and 12 months as well as in MS medium with various concentrations of sucrose<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> (4.5, 6, 9 and 12%) at 22°C. The material for long-term low temperature storage of the garlic samples in liquid nitrogen were the meristems of different development type, Duchess (winter) and Manuilivskyy (spring) varieties. The apices were dehydrated in plant<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> vitrification solution (1 M sucrose + 2 M glycerol + 2.5 M ethylene glycol) for 120 min, placed into cryovials and immersed into liquid<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> nitrogen. The samples were stored at the temperature of –196°C for 5 years. The storage at 4°C in 1/2 MS medium provided the<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> maximum preservation of the regenerated plants during a year (94.4%). During culturing at 22°C the highest viability (85%) was<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> observed in the medium, containig 12% sucrose. Long-term storage of garlic meristem at –196°C did not affect the viability and<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> growth rate if compared to the apices, thawed an hour after plunging into liquid nitrogen. The viability of vitrified-warmed garlic<span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"> meristems of Duchess and Manuilivskyy varieties was about 60%.</span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></p><p><span id="tinymce" class="mceContentBody " dir="ltr"><span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Arial,Italic; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"><span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"><em> </em><span style="font-family: Arial; font-size: 8pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Probl Cryobiol Cryomed 2017; 27(2): 110–120</em><br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" /></span></span></span></span></span></span></p> ER -