TY - JOUR AU - Plaksina, Ekaterina M. AU - Sidorenko, Olga C. AU - Bozhok, Galina A. PY - 2017/12/23 Y2 - 2024/03/28 TI - Cryopreservation of Multicellular Spheroids Derived From Newborn Piglet Adrenal Glands JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 27 IS - 4 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo27.04.322 UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1353 SP - 322-333 AB - <p>Application of special in vitro culture techniques for the cells, derived from different animal and human organs, makes<br />possible the obtaining of multicellular spheroids (MSs), being the natural 3-D environment for cells unlike the standard culture with the<br />cells in monolayer. Previously we have shown that MSs formed in the newborn piglet adrenal cell culture are capable to produce the<br />neuroblast-like cells, expressing the neuronal marker β-III-tubulin. In the present work we have assessed the regimens for MSs<br />cryopreservation using 5, 7 and 10% dimethyl sulfoxide (DMSO) and 25% fetal bovine serum (FBS). Analysis of some characteristics<br />of cryopreserved MSs (surface adhesion, capability to produce the neuroblast-like cells and monolayer formation by fibroblast-like<br />cells) allowed to choose the cryopreservation regimen with 1°C/min cooling rate in the presence of 10% DMSO as the most optimal<br />one. The FBS supplement to the medium did not significantly affect the cryopreservation outcome, although there was found a<br />tendency to increase the capability of cryopreserved MSs to produce the neuroblast- and fibroblast-like cells.</p><p><em>Probl Cryobiol Cryomed 2017; 27(4): </em><em>322-333</em></p> ER -