TY - JOUR AU - Sakun, O. V. PY - 2010/04/19 Y2 - 2024/03/28 TI - Cryoinjury Mechanisms in the Yeast Fungi Saccharomyces cerevisiae During Freezing in Dimethyl Sulfoxide Aqueous Solution at a Constant Rate in Cylindrical Containers JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 20 IS - 1 SE - Theoretical and Experimental Cryobiology DO - UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/217 SP - 59-65 AB - <p>The colony-forming ability of the yeast fungi <em>S. cerevisiae</em> suspended in 10% (vol. %) dimethyl sulfoxide – 0.135 M NaCl – water solution after freezing at the rates of 1, 5 and 10°C/min was determined. The method of multi-point thermography demonstrated the dependence of the average cooling rate in a frozen sample on the crystallisation stage of cell suspension on the coolant cooling rate in the cryostat and on the distance from the axles of cylindrical containers with the diameters of 10, 20 and 30 mm. The high colony-forming ability of the microorganisms <em>S. cerevisiae</em> was observed if a considerable amount of cells in a freezing container is cooled at the rates 4–8°C/min on the crystallization stage. The maximal colony-forming ability of the microorganisms <em>S. cerevisiae</em> was achieved if they were frozen in a container with the diameter of 20 mm at the coolant cooling rate of 5°C/min in the cryochamber.</p> ER -