TY - JOUR AU - Gorbunov, L. V. AU - Shiyanova, T. P. PY - 2009/11/30 Y2 - 2024/03/28 TI - Cryopreservation of Fruit-Berry Culture Cuttings JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 19 IS - 3 SE - Cryopreservation of Biological Resources DO - UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/257 SP - 338-348 AB - <p>It has been established that temperature, storage period, humidity, rates of cooling and thawing significantly affect viability of frozen-thawed cuttings. The found permissible values of humidity, parameters, providing maximal viability of birch, black currant berry, raspberry, cherry cuttings are 32–40; 40–50; 37–40; 33–45% within the positive range of temperatures and 14–28, 30–40, 23, 37% within the negative range. Decrease of humidity results in plasmolysis, and exceeding does in intracellular crystal-formation. During application of different regimens of drying and cooling with the rate of 0.1°C/hr down to –20°C with further plunging into liquid nitrogen the viability of frozen-thawed cuttings of currant berry and birch made 100%, 88% for raspberry and 57% for cherry.</p> ER -