TY - JOUR AU - Yakovleva, E. A. AU - Gerodes, A. G. PY - 2004/03/15 Y2 - 2024/03/29 TI - Effect of Low Temperature Storage on Steroid Hormones Content Stability in Human Oocyte Follicular Fluid JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 0 IS - 1 SE - Cryomedicine, Clinical and Experimental Transplantology DO - UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/709 SP - 83-88 AB - <p>There was studied the estradiol (E), progesterone (P) and testosterone (T) content in follicular fluid (FF) of human oocyte preovulatory follicles, native and low-temperature-stored one (-196°C). We have compared the effect of two freezing regimens (one-step immersion into liquid nitrogen and a two-step one with the preliminary exposure in nitrogen vapors) on the stability of concentration maintenance of these hormones in FF for 1 and 6 months. Stability of estradiol, progesterone and testosterone was shown to be kept within the whole observation period and to be independent upon the FL freezing regimen selected.</p> ER -