TY - JOUR AU - Babijchuk, Lyubov A. AU - Mykhailova, Olga A. AU - Ryazantsev, Vladimir V. AU - Zubov, Pavel M. AU - Migunova, Regina K. PY - 2016/03/21 Y2 - 2024/03/29 TI - Cryopreservation of Cord Blood Nucleated Cells Using Non-Penetrating Cryoprotectant PEO-1500 JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 26 IS - 1 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo26.01.024 UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/813 SP - 24-34 AB - <p>Development of wash-free cryopreservation methods for cord blood nucleated cell fraction, including the population of hematopoietic stem/progenitor cells, is an actual task for their long-term storage. Present investigation involved the technique of cord blood nucleated cells cryopreservation, comprising the isolation of cell population by the original two-step centrifugation method, the treatment with non-penetrating cryoprotectant polyethylene oxide with molecular weight of 1500 (PEO-1500) and freezing by the two-step program. This technique enabled to preserve more than 72% CD45+ and 75% CD34+ cells with the viability indices of correspondingly 83 and 91% and higher. Herewith, an increased content of reactive oxygen species (42.7 ± 6.3%) on the background of high cell number and their viability may be considered as a cells’ physiological response to the effect of cryopreservation factors.</p><p><em><br /></em></p><p><em>Probl Cryobiol Cryomed 2016; 26(1):24-34.</em></p> ER -