TY - JOUR AU - Khudyakov, Andrey N. AU - Polezhaeva, Tatyana V. AU - Zaitseva, Oksana O. AU - Laptev, Denis S. AU - Solomina, Olga N. AU - Kostyaev, Andrey A. PY - 2013/03/15 Y2 - 2024/03/28 TI - Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 23 IS - 1 SE - Theoretical and Experimental Cryobiology DO - UR - https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/9 SP - 49-57 AB - <p>Using chemiluminescence method it was shown that after addition of several cryoprotectants 1,2-propane diol (21% initial concentration hereinafter and added 1:1) and dimethyl sulfoxide (8%) to leukocyte suspension the level of LPO and antioxidant activity decreased. Presence of glycerol (7%) and hexa-methylene bis-tetra hydroxyethyl urea (30%) resulted in simultaneous stimulation of LPO and antioxidant systems. Hydroxyethylated starch (5.7%) and gelatine (molecular weight of 16,000; 7.5%) increase LPO. After storage in combined cryoprotective media at –10, –20 and –40°C the leukocytes revealed the activation of antioxidant systems on the background of stable LPO indices, that, probably, contributed to morphofunctional preservation of 75% of cells.<i>Probl Cryobiol Cryomed 2013; 23(1):49–57.</i></p> ER -