Problems of Cryobiology and Cryomedicine

Cover of the issue

2026-03-28T15:48:40+01:00

Editorial information

2026-03-28T17:01:55+01:00

Table of Content

2026-03-28T17:10:42+01:00

Probl Cryobiol Cryomed 2025; 35(4): 165-166

Modeling cell osmotic behavior for improvement of cryopreservation protocol

2026-03-08T20:58:10+01:00

Cryopreservation of cell suspensions, such as testicular interstitial cells (ICs), which include cells differing in size, volume, and membrane composition, is a very challenging task. Cryobiology requires the determination of several parameters to optimize the cryopreservation procedure, such as membrane water permeability (or hydraulic conductivity) (Lp) and its activation energy (Ea0), the permeability of the cell plasma membrane to dimethyl sulfoxide (DMSO) (Ps) and its activation energy (Ea1), and the osmotically inactive cell volume. This study aims to measure these key cryopreservation parameters for ICs. Additionally, it seeks to evaluate how quickly ICs become saturated with DMSO and to determine the optimal exposure time to cryoprotective media before cooling. By analyzing cell volume changes in different DMSO-based solutions, the research intends to optimize the cryopreservation process, potentially eliminating unnecessary exposure steps while maintaining cell viability. The kinetics of ICs volume changes in cryoprotective media with different DMSO concentrations (0.7—2.8 M), which additionally included 100 mg/ml dextran 40 (0.7DMSO + D40) or 10% FBS (1.4DMSO + FBS), were analyzed using the modified Kedem–Katchalsky model to determine the membrane transport properties. The calculated parameters clearly showed that cell saturation with DMSO occurred within a few minutes after its addition and could reach equilibrium before the estimated start of water crystallization in the samples. Thus, additional exposure of cells to DMSO-containing media was unnecessary. It could potentially lower the metabolic activity of ICs. Accordingly, the cryopreservation procedure for ICs can be shortened by removing the exposure step without reducing viability of ICs in 0.7DMSO + D40 and 1.4DMSO + FBS.

Probl Cryobiol Cryomed. 2025; 35(4): 194—207

Assessment of hyaluronic acid and DMSO influence on low-temperature tissue damage course: an experimental model

2026-03-08T21:14:50+01:00

The research performed a local histological assessment of tissue tolerance of a solution containing hyaluronic acid, as well as its effect on the course of the inflammatory process aft er low-temperature skin damage was investigated. The safety of using a 1% solution of high molecular weight hyaluronic acid with the addition of 5% dimethyl sulfoxide (DMSO) and its effect on the inflammation development was studied using a model of low-temperature tissue injury based on the results of clinical observation, planimetric and histological studies. Additionally, the effect of 1% solutions of high molecular weight (> 2,000 kDa) and low molecular weight (10—100 kDa) hyaluronic acid on the inflammatory process aft er low-temperature tissue injury was comparatively explored. According to the results of the histological study, no morphological signs of toxic or other negative effects of subcutaneous administration of a 1% solution of high molecular weight hyaluronic acid with the addition of 5% DMSO on tissues were found. Comparison of the effects of cryoprotective solutions on the course of the wound process after low-temperature tissue damage revealed the differences both by the severity of macroscopic signs of the inflammation and by planimetric study data. The inclusion of hyaluronic acid in the composition of the cryoprotective solution is considered a optimistic approach to reducing the concentration of DMSO in order to create solutions that can be used without a washing-out stage.

Probl Cryobiol Cryomed. 2025; 35(4): 208—218

Hypoxia as a modulating factor of human sperm capacitation and cryoresistance

2026-03-08T21:31:53+01:00

Despite increasing interest to the role of hypoxia in reproductive processes, its impact on sperm capacitation and cryoresistance remains insufficiently described. This study investigated the effect of oxygen tension during pre-cryopreservation sperm incubation on the capacitation efficiency and examined the relationship between capacitation and cryoresistance. According to the WHO criteria, ejaculates from 30 healthy normozoospermic men were analyzed. Functional characteristics and cryoresistance of spermatozoa were assessed after density gradient processing and in vitro capacitation under 1, 5 and 21% oxygen concentrations. An incubation of human spermatozoa under moderate hypoxia was found to positively influence the viability, motility, and capacitation activation. For the first time, we have demonstrated that oxygen tension during in vitro incubation is a critical factor determining sperm survival following thawing. These findings suggest that moderate hypoxia may represent a promising biotechnological strategy to improve sperm cryopreservation protocols and enhance the efficiency of assisted reproductive technologies.

Probl Cryobiol Cryomed. 2025; 35(4): 219—223

Study on the dependence of alpha-fetoprotein content in human cord blood extracts on extraction conditions

2026-03-08T21:42:37+01:00

Probl Cryobiol Cryomed. 2025; 35(4): 224—226

In memoriam of Leonid F. Rozanov

2026-03-08T21:56:25+01:00

Probl Cryobiol Cryomed. 2025; 35(4): 229

In memoriam of Vladyslav G. Babiychuk

2026-03-08T22:01:09+01:00

Probl Cryobiol Cryomed. 2025; 35(4): 230

Backmatter

2026-03-28T17:20:50+01:00

Instruction for Authors

2026-03-28T17:26:07+01:00

Probl Cryobiol Cryomed 2025; 35(4): 253-254

Key Words Index, Year 2025, Volume 35

2026-03-28T17:32:08+01:00

Probl Cryobiol Cryomed 2025; 35(4):255-256

Table Of Contents, Year 2025, Volume 35

2026-03-28T17:38:52+01:00

Probl Cryobiol Cryomed 2025; 35(4):257-258

Biocryoimmunology — a new scientific revelation in bioscience and medical practice

2026-03-08T22:03:24+01:00

Probl Cryobiol Cryomed 2025; 35(4):231

Wharton’s jelly tissue cryopreservation for efficient mesenchymal stromal/stem cell retrieval

2026-03-08T22:15:55+01:00

Probl Cryobiol Cryomed 2025; 35(4):231

Cryopreservation of equine and canine monocyte derived dendritic cells

2026-03-08T22:22:50+01:00

Probl Cryobiol Cryomed 2025; 35(4):232

Cryopreservation of fish embryos: insights from Cyprinodon variegatus

2026-03-08T22:37:21+01:00

Probl Cryobiol Cryomed 2025; 35(4):232

Vitrification of male gametes — our experience

2026-03-08T22:45:10+01:00

Probl Cryobiol Cryomed 2025; 35(4):233

Enhancing seed supply in mussel aquaculture: a cryopreservation study using two cooling systems

2026-03-08T22:51:03+01:00

Probl Cryobiol Cryomed 2025; 35(4):233

Cryoresistance and potential vitrification in lichen thalli

2026-03-08T22:59:39+01:00

Probl Cryobiol Cryomed 2025; 35(4):234

Optimization of the conditions for the formation and cryopreservation of compatible spheroids of rat mesenchymal stem cells and neural cells

2026-03-08T23:14:38+01:00

Probl Cryobiol Cryomed 2025; 35(4):234

Engineered microenvironments for MSCs storage at ambient temperature in alginate core-shell capsules

2026-03-09T08:37:28+01:00

Probl Cryobiol Cryomed 2025; 35(4):235

Effect of pre-chilling on ovarian tissue

2026-03-09T09:29:23+01:00

Probl Cryobiol Cryomed 2025; 35(4):235

Cryotechnologies in animal breeding: EU experience and opportunities for Ukraine ̉s integration

2026-03-09T10:12:59+01:00

Probl Cryobiol Cryomed 2025; 35(4):236

Enhancing cryopreservation of organoids via diffusion kinetics analysis using Cryo-Raman microspectroscopy

2026-03-09T10:33:05+01:00

Probl Cryobiol Cryomed 2025; 35(4):236

Cell-tissue therapy of fetoplacental origin in recurrent implantation failure and chronic endometritis: a clinical case report

2026-03-09T10:52:39+01:00

Probl Cryobiol Cryomed 2025; 35(4):237

Design of a fluidic chamber for the analysis of diffusion CPA/VA in a single cell using Raman microscopy

2026-03-09T11:11:29+01:00

Probl Cryobiol Cryomed 2025; 35(4):237

Confocal microscopic imaging of sperm cells: a step toward optimised directional freezing protocols

2026-03-09T11:39:00+01:00

Probl Cryobiol Cryomed 2025; 35(4):238

From traditional to sustainable: new materials for polymer electrolyte membranes

2026-03-09T12:01:35+01:00

Probl Cryobiol Cryomed 2025; 35(4):238

Dependence of posthypertonic hemolysis of initial state of canine erythrocytes

2026-03-09T12:07:34+01:00

Probl Cryobiol Cryomed 2025; 35(4):239

Encapsulation-based strategies for enhanced ovarian vitrification: experimental design and research rationale

2026-03-09T12:19:19+01:00

Probl Cryobiol Cryomed 2025; 35(4):239

Possible use of sucrose for pretreatment and subsequent cryopreservation of newborn rats’ brain-derived neural cells

2026-03-09T12:23:04+01:00

Probl Cryobiol Cryomed 2025; 35(4):240

The influence of the implant of cryopreserved placenta tissue on morphofunctional state of recipient’s placenta

2026-03-09T12:28:46+01:00

Probl Cryobiol Cryomed 2025; 35(4):240

Application of cryopreserved umbilical cord blood and placenta-derived products in the treatment of infertility in a patient with diminished ovarian reserve. Case report

2026-03-09T12:32:39+01:00

Probl Cryobiol Cryomed 2025; 35(4):241

Biochemical markers of cholestasis in rats with acetaminophen-induced intoxication after partial hepatectomy

2026-03-09T13:17:12+01:00

Probl Cryobiol Cryomed 2025; 35(4):241

Efficacy of cryotherapy in recovery from muscle injuries: a systematic review

2026-03-09T13:56:08+01:00

Probl Cryobiol Cryomed 2025; 35(4):242

Impact of hyaluronic acid on healing of cold wounds

2026-03-09T14:00:37+01:00

Probl Cryobiol Cryomed 2025; 35(4):242

The effect of intermittent cold exposure on the level of reactive oxygen species production in erythrocytes of rats with induced polycystic ovary syndrome

2026-03-09T14:07:00+01:00

Probl Cryobiol Cryomed 2025; 35(4):243

Study of the permeability of the plasma membrane of human erythrocytes for DMSO molecules in the presence of PVA of various concentrations

2026-03-09T14:12:50+01:00

Probl Cryobiol Cryomed 2025; 35(4):243

Effect of synthetic leu-enkephalin on the lipid profile in homeothermic animals under conditions of chronic cold stress

2026-03-09T18:54:41+01:00

Probl Cryobiol Cryomed 2025; 35(4):244

Membrane destabilization by Gramicidin S: evaluating its compatibility with cryoprotective protocols

2026-03-09T19:26:45+01:00

Probl Cryobiol Cryomed 2025; 35(4):244

Evaluation of the impact of hypoxic conditions on the viability of spermatozoa after capacitation and cryopreservation

2026-03-09T19:30:41+01:00

Probl Cryobiol Cryomed 2025; 35(4):245

Fish reproductive cells collection of the Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine: history, significance and perspectives

2026-03-09T19:37:51+01:00

Probl Cryobiol Cryomed 2025; 35(4):245

Study of the physical properties of hydrogel films for low-temperature storage of microorganisms

2026-03-09T19:45:05+01:00

Probl Cryobiol Cryomed 2025; 35(4):246

Hormonal stimulation of silver crucian carp using a combination of surfagon (GnRH) and metoclopramide for the reintroduction of rare fish species based on cryobank resources

2026-03-09T19:49:37+01:00

Probl Cryobiol Cryomed 2025; 35(4):246

Evaluation of the effect of various cryoprotectants on the resistance of canine erythrocytes to mechanical stress

2026-03-09T19:54:36+01:00

Probl Cryobiol Cryomed 2025; 35(4):247

Cryopreservation of oocytes as a method of implementing reproductive plans in women with cervical cancer

2026-03-09T20:02:08+01:00

Probl Cryobiol Cryomed 2025; 35(4):247

Lipid profile of spontaneously hypertensive rats after application of rhythmic extreme cryoeffect and conditioned medium of mesenchymal stromal cells

2026-03-09T20:13:34+01:00

Probl Cryobiol Cryomed 2025; 35(4):248

Temperature-adapted invasive species of aquarium invertebrates as a threat to the local fauna of Kharkiv Region (Ukraine)

2026-03-09T20:17:28+01:00

Probl Cryobiol Cryomed 2025; 35(4):248

Biological activity of an aqueous solution of fullerene C60 molecules

2026-03-09T20:20:09+01:00

Probl Cryobiol Cryomed 2025; 35(4):249

Effect of different yolk treatments on cold resistance of boar semen

2026-03-09T20:24:02+01:00

Probl Cryobiol Cryomed 2025; 35(4):249

Application of cryopreserved placental polypeptide complex in murine acubarotrauma model

2026-03-09T20:47:56+01:00

Probl Cryobiol Cryomed 2025; 35(4):250

The role of nanobiotechnology in improving cryopreservation methods for cells and tissues

2026-03-09T21:00:05+01:00

Probl Cryobiol Cryomed 2025; 35(4):250

Abstract Index

2026-03-28T18:20:47+01:00

Probl Cryobiol Cryomed 2025; 35(4):251-252

Legal and bioethical foundations of human cell and tissue cryobanks in Ukraine: regulatory framework, challenges and prospects

2026-03-08T19:34:19+01:00

The paper considers the legal and bioethical foundations of the activity of cryobanks of human cells and tissues in Ukraine. The importance of adapting national legislation to international standards in the field of biobanking, transplantology and assisted reproductive technologies is shown. The study provides an analysis of the basics of legal regulation of cryobanks, identifies directions for improving the legislation of Ukraine in accordance with European standards. For the analysis, the authors were guided by the laws of Ukraine, directives of the European Union, recommendations of the World Health Organization, documents of the Council of Europe, and used scientific publications in the field of medical law, bioethics, cryobiology, and reproductive medicine. Regulatory, documentary, comparative methods of analysis were applied. It has been established that the current regulatory framework for governing the activities of cryobanks is fragmented, does not determine the legal status of biological material, the procedure for its inheritance and use after the donor’s death. The proposed ways to improve the legal field are as follows: adoption of a special law on biobanks, creation of a unified state register of biological samples, adaptation of protocols and procedures to the norms of the European Union. The implementation of the proposed measures will contribute to the transparency, ethics, and efficiency of cryobanks, the protection of the rights of donors and patients, and the development of biomedical research in Ukraine.

Probl Cryobiol Cryomed. 2025; 35(4): 165—178

Use of methods for determining cell viability in cryobiological research

2026-03-08T20:47:01+01:00

Assessment of cell viability is critically important in the context of cryobiological research, as it allows determining the effectiveness of freeze-thawing procedures and low-temperature storage of isolated cells. The review systematizes the methods for determining cell viability used in cryobiological research, with an emphasis on their practical applicability, accuracy, and sensitivity. Both traditional methods based on the assessment of cell membrane integrity and metabolic activity, such as trypan blue staining, MTT assay, fluorescent dye assays, and more modern approaches, including flow cytometry, confocal microscopy, and methods for assessing cell functional activity, are reviewed. For each method, a description of the mechanism of action, a protocol, specific advantages, disadvantages, as well as equipment requirements and limitations for cell types are provided. A comparative analysis was conducted, which revealed the lack of a universal approach, but demonstrated the effectiveness of combined methods that combine fluorescent staining and metabolic assessment. Such combinations allow for high accuracy in assessing cell viability after cryopreservation. In addition, the review discusses the prospects for the development of methods for determining cell viability in cryobiology, including the introduction of the latest high-resolution imaging technologies, multiparametric analysis, as well as integration with microfluidic platforms and artificial intelligence to automate and improve the accuracy of the assessment. The review can serve as a practical tool for researchers in choosing the most optimal approach for assessing cell viability depending on the purpose of the experiment, available resources and cell type.

Probl Cryobiol Cryomed. 2025; 35(4): 179—193