Ultrastructure of Nucleated Cells of Cord Blood and Blood of Adult Donors at Different Stages of Cryopreservation. Report 1. The Changes on the Stage of Cells Isolation

Authors

  • L. A. Babiychuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • R. K. Migunova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. F. Nevzorova Institute of General and Emergency Surgery Medical Sciences of Ukraine, Kharkov
  • V. P. Nevzorov Institute of General and Emergency Surgery Medical Sciences of Ukraine, Kharkov

Keywords:

nucleated cells, adult donor blood, cord blood, ultrastructure

Abstract

Ultrastructure characteristics were studied in the nucleated cells from adult donor blood and cord blood isolated by different methods. Electron microscopical investigation revealed quite good preservation of characteristic ultrastructure in nucleated cells of adult donor blood and cord blood isolated by two-step centrifugation and sedimentation in Poliglyukin. Presence of large amount of ribosomes and polysomes in cytoplasm testifies to a high activity of synthesizing and reparative function of the cells. Isolation of nucleated cells from adult donor blood and cord blood using Ficoll leads to destructions of membrane structures and organelles, which impedes the restoration of normal metabolic activity by intracellular reparation.

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Published

2012-12-20

How to Cite

Babiychuk, L. A., Migunova, R. K., Nevzorova, O. F., & Nevzorov, V. P. (2012). Ultrastructure of Nucleated Cells of Cord Blood and Blood of Adult Donors at Different Stages of Cryopreservation. Report 1. The Changes on the Stage of Cells Isolation. Problems of Cryobiology and Cryomedicine, 22(3), 453–461. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/49

Issue

Section

Cryopreservation of Biological Resources