Peculiarities of Hypertonic Cryohemolysis and Posthypertonic Lysis of Human Erythrocytes under Phenobarbital and Barbital Effect

Authors

  • V. A. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. V. Prokopenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocyte, barbital, phenobarbital, hemolysis, posthypertonic lysis, hypertonic cryohemolysis, dehydration

Abstract

Dynamics of hypertonic cryohemolysis and posthypertonic lysis of human erythrocytes was calorimetrically studied along with the introduction of barbital and phenobarbital in different concentrations into incubation medium. Barbital and phenobarbital were shown not to change the level of posthypertonic lysis when erythrocytes hemolysis occurred as a result of hemolytic pore formation in the rehydration phase. During hypertonic cryohemolysis the osmotic and temperature factors are combined; erythrocytes damage is influenced not only by dehydration, but also by ionic transport. Barbital and phenobarbital decrease the level of hypertonic cryohemolysis along with the decrease of ions transport through erythrocytes membrane.

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Published

2005-03-22

How to Cite

Bondarenko, V. A., & Prokopenko, N. V. (2005). Peculiarities of Hypertonic Cryohemolysis and Posthypertonic Lysis of Human Erythrocytes under Phenobarbital and Barbital Effect. Problems of Cryobiology and Cryomedicine, 15(1), 27–32. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/642

Issue

Vol. 15 No. 1 (2005): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. A. Bondarenko, N. V. Prokopenko

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