Effect of Storage Temperature Variations on Viability of Cryopreserved Cells of Pro- and Eukaryotes

Authors

  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. V. Kudokotseva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. F. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. I. Groshevoy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. F. Martsenyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Koshchiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, temperature gradients, storage, cells, low temperature bank

Abstract

There was experimentally found the probability of extradeath of microorganism, human embryos’ and tumour cells when varying the temperature from –196 up to –130 and –100°C. There was studied the dynamics of temperature change in ‘Nunc’ cryotubes with cell suspensions during technological operations in low temperature bank of biological objects.

Downloads

Published

2005-03-22

How to Cite

Vysekantsev, I. P., Kudokotseva, O. V., Petrenko, T. F., Gurina, T. M., Groshevoy, M. I., Martsenyuk, V. F., & Koshchiy, S. V. (2005). Effect of Storage Temperature Variations on Viability of Cryopreserved Cells of Pro- and Eukaryotes. Problems of Cryobiology and Cryomedicine, 15(1), 33–41. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/643

Issue

Section

Theoretical and Experimental Cryobiology